• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>World multi-conference on systemics, cybernetics and informatics >Effect of Surface Morphology of Scaffold with Lines of Micro Ridges on Deformation of Cells
【24h】

Effect of Surface Morphology of Scaffold with Lines of Micro Ridges on Deformation of Cells

机译：微脊线支架的表面形态对细胞变形的影响

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The effect of micro ridges of the scaffold on deformation of cells has been studied in vitro. Several parallel lines of micro ridges have been made on a disk of transparent polydimethylsiloxane. The height, the width, and the length of the rectangular ridge are 0.001 mm, 0.003 mm, and 0.5 mm, respectively. Variation has been made on the interval of the ridge: 0.001 mm, 0.003 mm, 0.005 mm and 0.007 mm. Five kinds of cells were used in the test. C2C12 (mouse myoblast cell line), L929 (fibroblast connective tissue of mouse), Neuro-2a (a mouse neural cell line), 3T3-L1 (mouse fat precursor cells), and HUVEC (human umbilical vein endothelial cells). Cells were seeded on the micro pattern at the density of 2000 cells/cm~2 and cultured for 24 hours. The cells were observed with a microscope. The experimental results show that C2C12 lengthen along the line of ridge of 0.003 mm interval. The effects of the micro pattern depend on the kind of cells.
机译：在体外研究了支架的微脊对细胞变形的影响。在透明的聚二甲基硅氧烷的圆盘上制作了几条平行的微脊线。矩形脊的高度，宽度和长度分别为0.001mm，0.003mm和0.5mm。凸脊的间距有所不同：0.001毫米，0.003毫米，0.005毫米和0.007毫米。测试中使用了五种电池。 C2C12（小鼠成肌细胞细胞系），L929（小鼠成纤维细胞结缔组织），Neuro-2a（小鼠神经细胞系），3T3-L1（小鼠脂肪前体细胞）和HUVEC（人脐静脉内皮细胞）。将细胞以2000个细胞/ cm 2的密度接种在微图案上，并培养24小时。用显微镜观察细胞。实验结果表明，C2C12沿0.003 mm间隔的脊线延长。微观图案的效果取决于细胞的种类。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号