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首页> 外文会议>International Conference on Advanced Robotics and Mechatronics >The establishment of detecting carbohydrate antigen 153 (CA153) with immunofluorescence chromatography
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The establishment of detecting carbohydrate antigen 153 (CA153) with immunofluorescence chromatography

机译：免疫荧光色谱法检测碳水化合物抗原153（CA153）的建立

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This study was designed to develop a immunofluorescence chromatography kit for detection of CA153 in human serum, and to evaluate the method performance. The fluorescent polystyrene particle conjugated with the monoclonal antibody MIC15 was used as a fluorescent label. The monoclonal antibody MIC16 and mouse anti-rabbit IgG were immobilized on the nitrocellulose membrane as the test line and control line. The linear response rang of the method to detect the serum CA153 was 0.36-250U/ml. And the intra-and inter-assay coefficient were 5.81% and 9.88%. Total 1200 samples were tested by the immunofluorescence chromatography kit, and the overall coincidence rate was 96.35%. The positive coincidence rate was 95.62%, the negative coincidence rate was 97.23%, and the Kappa value surpassed 0.8, which indicated that there was no significant difference between the results of the immunofluorescence chromatography kit and of isolated culture. In conclusion, the immunofluorescence chromatography kit for assaying CA153 provided high sensitivity, rapid, simple operation, low cost, single-servings and can be extensively used for clinical operarions.
机译：本研究旨在开发一种用于检测人血清中CA153的免疫荧光色谱试剂盒，并评估该方法的性能。与单克隆抗体MIC15缀合的荧光聚苯乙烯颗粒用作荧光标记。将单克隆抗体MIC16和小鼠抗兔IgG固定在硝酸纤维素膜上作为测试线和对照线。检测血清CA153的方法的线性响应范围为0.36-250U / ml。批内和批间系数分别为5.81 \％和9.88 \％。用免疫荧光色谱试剂盒检测了1200个样品，总符合率为96.35％。阳性符合率为95.62％，阴性符合率为97.23％，Kappa值超过0.8，表明免疫荧光层析试剂盒结果与分离培养物之间无显着差异。综上所述，用于检测CA153的免疫荧光色谱试剂盒具有高灵敏度，快速，简单操作，成本低，一次性进样的优点，可广泛用于临床手术。

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