• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>IEEE International Conference on Industrial Engineering and Applications >In Vitro Experiments of Microwave Ablation in Liver Cancer Cells (Effects of Microwave Power and Heating Time)
【24h】

In Vitro Experiments of Microwave Ablation in Liver Cancer Cells (Effects of Microwave Power and Heating Time)

机译：肝癌细胞微波消融的体外实验（微波功率和加热时间的影响）

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microwave ablation (MWA) is new promising alternative liver cancer treatment. It is a modality that use microwave energy to induce heat and then increase temperature within cancerous tissue to kill liver cancer cells. The cancer cell temperature of 50°C is required as starting point to induce cell death. Important parameters of MWA include microwave power, heating time as well as temperature achieved during the process. All of them affects effectiveness of this modality on killing cancer cells. In this research, in vitro experiment of MWA in liver cancer cells cultured (HepG2) is carried out. Cell viability of liver cancer cells then is measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). Temperature distribution at various four points within cultured liver cancer cells is recorded during MWA. At the end of MWA, surface temperature profile of liver cancer cells is taken. Liver cancer cells undergo MWA based on microwave power of 5, 10 and 20 W for heating time of 10 min and microwave power of 20 W for heating time of 3, 5 and 10 min. Effects of microwave power and heating time on cell viability, temperature distribution as well as surface temperature profile of liver cancer cells are investigated. The results show that microwave power and heating time have the effect on cell viability significantly. Overall significant difference of cell viability is observed for each microwave power and heating time setting. Cell viability decreases with increasing microwave power and heating time. In addition, temperature distribution and surface temperature profile increase with increasing microwave power and heating time. Additionally, it is observed that temperature of liver cancer cells lasted for proper heating time is necessary to cause cell death. When the temperature reaches the target at 50°C for proper some time, MWA has efficiency in killing more than 50% of liver cancer cells. This research provides the essential aspects for planning cancer treatment using MWA in clinic.
机译：微波消融（MWA）是新的有前途的替代肝癌治疗方法。它是一种利用微波能量诱导热量，然后升高癌组织内温度杀死肝癌细胞的方法。需要50℃的癌细胞温度作为诱导细胞死亡的起点。 MWA的重要参数包括微波功率，加热时间以及过程中达到的温度。所有这些都影响这种方法杀死癌细胞的有效性。在这项研究中，在培养的肝癌细胞（HepG2）中进行了MWA的体外实验。然后使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物测定法（MTT测定法）测量肝癌细胞的细胞生存力。在MWA期间记录培养的肝癌细胞内各个四个点的温度分布。在MWA结束时，获取肝癌细胞的表面温度曲线。肝癌细胞根据微波功率5 W，10 W和20 W，加热时间10分钟以及微波功率20 W，3、5和10分钟的加热时间进行MWA。研究了微波功率和加热时间对肝癌细胞活力，温度分布以及表面温度分布的影响。结果表明，微波功率和加热时间对细胞活力有显着影响。对于每种微波功率和加热时间设置，观察到细胞活力的总体显着差异。细胞活力随着微波功率和加热时间的增加而降低。另外，温度分布和表面温度分布随微波功率和加热时间的增加而增加。另外，观察到持续适当加热时间的肝癌细胞的温度对于引起细胞死亡是必要的。当温度达到目标温度50°C适当的时间后，MWA可以杀死50％以上的肝癌细胞。这项研究提供了计划在临床中使用MWA进行癌症治疗的基本方面。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号