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首页> 外文会议>Engineering in Medicine and Biology Society, 1998. Proceedings of the 20th Annual International Conference of the IEEE >In vitro monitoring of cytochrome oxidase activity in neuronal cells
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In vitro monitoring of cytochrome oxidase activity in neuronal cells

机译：体外监测神经元细胞中细胞色素氧化酶活性

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The purpose of this investigation is to design a system to measure cellular activity in relation to the activity of cytochrome c oxidase (Cox), a key metabolic enzyme found in all cell types. Cox levels in neuronal cells have been found to be high when compared to other cell types. This system is being developed to investigate cell metabolism of neurons growing in a micro-machined silicon glass chamber designed for studying neuronal growth. Cox has also been linked with neurodegenerative disorders such as Alzheimer's and Parkinson's disease and may have a role as a marker for such diseases. Here, the authors explore the possibility of using NIRS (Near Infrared Spectroscopy) 845 nm region using FTIR (Fourier Transform Infra-Red) spectroscopy. Changes in the oxidation state of Cox can be seen as changes in absorption at 845 nm. As part of this investigation, quantification and analysis of Cox was also carried out using Western blot protein analysis, immunochemistry and fluorescence microscopy to detect levels in neuroblastoma (NG115-401 L) and primary rat neuronal cells.
机译：这项研究的目的是设计一种系统，以测量与细胞色素C氧化酶（Cox）的活性有关的细胞活性，Cox是在所有细胞类型中发现的关键代谢酶。与其他细胞类型相比，已发现神经元细胞中的Cox水平较高。正在开发该系统以研究在设计用于研究神经元生长的微加工硅玻璃腔室内生长的神经元的细胞代谢。考克斯还与神经退行性疾病如阿尔茨海默氏病和帕金森氏病有关，并可能作为此类疾病的标志物。在这里，作者探索了通过FTIR（傅里叶变换红外）光谱仪在845 nm区域使用NIRS（近红外光谱仪）的可能性。 Cox氧化态的变化可以看作是845 nm处吸收的变化。作为这项研究的一部分，还使用蛋白质印迹蛋白分析，免疫化学和荧光显微镜术对Cox进行了定量和分析，以检测神经母细胞瘤（NG115-401 L）和原代大鼠神经元细胞中的水平。

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