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首页> 外文会议>ASME microanoscale heat and mass transfer international conference;MNHMT2009 >THE ROLE OF ION ELECTROPHORESIS IN ELECTROPORATION-MEDIATED MOLECULAR DELIVERY
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THE ROLE OF ION ELECTROPHORESIS IN ELECTROPORATION-MEDIATED MOLECULAR DELIVERY

机译：离子电泳在电介导的分子传递中的作用

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Electroporation is a widely applied technique to deliver active molecules into the cellular compartment, to perform tasks such as gene therapy and directed stem cell differentiation, among many others. In this technique, an electric field transiently permeabilizes the cellular membrane to facilitate molecular exchange. While the permeabilization process is relatively well understood, the transport mechanisms for molecular delivery are still under debate. In this work, the role of ion electrophoresis in electroporation-mediated molecular delivery is investigated using numerical simulation. The Nernst-Planck equations for ionic transport in the extracellular and intracellular spaces are solved, respectively, and are coupled through a permeabilization model on the membrane. For the latter, an asymptotic Smoluchowski equation system is adopted, following the work of Krassowska and co-authors. The simulation is used to investigate the delivery of calcium ions into Chinese hamster ovary cells. The results indicate that ion electrophoresis is the dominant mode of transport in the delivery of small charged molecules. Furthermore, the achievable intracellular concentration is strongly influenced by the conductivity difference between the cytoplasm and the buffer, a phenomenon known as "field-amplified sample stacking". The results agree qualitatively with the fluorescence measurements by Gabriel and Teissie (1999), and suggest a new possibility to simultaneously improve cell viability and efficiency in electroporation-mediated molecular delivery.
机译：电穿孔是一种广泛应用的技术，可以将活性分子传递到细胞室内，以执行诸如基因治疗和定向干细胞分化等任务。在该技术中，电场使细胞膜瞬时渗透，以促进分子交换。尽管透化过程相对被很好地理解，但分子递送的转运机制仍在争论中。在这项工作中，使用数值模拟研究了离子电泳在电穿孔介导的分子传递中的作用。分别求解了细胞外和细胞内空间中离子迁移的Nernst-Planck方程，并通过膜上的通透模型进行耦合。对于后者，在Krassowska及其合作者的工作之后，采用了渐近Smoluchowski方程系统。该模拟用于研究钙离子向中国仓鼠卵巢细胞中的递送。结果表明，离子电泳是小电荷分子传递中的主要运输方式。此外，可达到的细胞内浓度受细胞质和缓冲液之间电导率差异的强烈影响，这种现象称为“现场扩增样品堆叠”。结果与Gabriel和Teissie（1999）的荧光测量结果在质量上吻合，并提出了一种新的可能性，可以同时提高细胞活力和电穿孔介导的分子传递效率。

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