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首页> 外文会议>2010 4th International Conference on Bioinformatics and Biomedical Engineering >Biotransformation of Sodium L-Glutamate to gamma-Aminobutyric Acid by L. Brevis TCCC13007 with Two Glutamate Decarboxylase Genes
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Biotransformation of Sodium L-Glutamate to gamma-Aminobutyric Acid by L. Brevis TCCC13007 with Two Glutamate Decarboxylase Genes

机译：带有两个谷氨酸脱羧酶基因的L. Brevis TCCC13007将L-谷氨酸钠生物转化为γ-氨基丁酸

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摘要

Due to the important physiological functions of GABA, various methods used to produce it. Biotransformation by microorganism cells is a more promising method than others. Among the GABA-producing lactic acid bacteria, one strain with high producing capacity was isolated from Chinese naturally pickled vegetable. The strain is identified as Lactobacillus brevis, and named as L.brevis TCCC13007 based on its physiological and biochemical characteristics and homology of 16S rDNA. In this study, two gad genes were cloned by PCR using the genomic DNA of L.brevis TCCC13007 as template. The deduced amino acid sequence of one gad gene showed 100%, 98.5% and 95.9% identity of L.brevis ATCC367, L. brevis BH2 and L. brevis OPK-3, respectively. The other showed 99.8% and 99.4% identity of L.brevis ATCC367 and L.brevis IFO12005respectively. According to the properties of GAD, the optimal biotransformation condition was investigated. Under the optimal condition, L.brevis TCCC13007 synthesised GABA at a concentration of 39 g.L-1.
机译：由于GABA具有重要的生理功能，因此可以使用多种方法生产GABA。微生物细胞的生物转化是一种比其他方法更有前景的方法。在生产GABA的乳酸菌中，从中国的天然腌制蔬菜中分离出一种具有高生产能力的菌株。基于其生理生化特性和16S rDNA的同源性，该菌株被鉴定为短乳杆菌，并被命名为短乳杆菌TCCC13007。在这项研究中，以短乳杆菌TCCC13007的基因组DNA为模板，通过PCR克隆了两个gad基因。一个gad基因的推导氨基酸序列与短乳杆菌ATCC367，短乳杆菌BH2和短乳杆菌OPK-3分别具有100％，98.5％和95.9％的同一性。另一对分别显示L.brevis ATCC367和L.brevis IFO12005的99.8％和99.4％身份。根据GAD的性质，研究了最佳的生物转化条件。在最佳条件下，L.brevis TCCC13007合成了39 g.L-1的GABA。

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