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首页> 外文会议>2010 3rd International Conference on Biomedical Engineering and Informatics >Molecular cloning and characterization analysis of LEF-1 gene from Inner Mongolia Cashmere Goat
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Molecular cloning and characterization analysis of LEF-1 gene from Inner Mongolia Cashmere Goat

机译：内蒙古绒山羊山羊LEF-1基因的分子克隆与特征分析

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LEF-1, lymphocyte enhancer-binding factor-1, is a member of the LEF-1/TCF (T cell-specific transcription factor) family of high mobility group (HMG) transcription factors. LEF-1/TCF is downstream of the Wnt/β-catenin signaling pathway. LEF-1 requires Wnt signaling and stabilized β-catenin to express the hair-specific keratin genes and control hair differentiation. Tcf3/LEF-1 complex has a key role in controlling skin stem cells differentiation. Thus this study amplified LEF-1 cDNA by RTPCR from Inner Mongolia Cashmere Goat (Capra hircus) to produce a transgenic goat with increased cashmere production. The molecular characterization of the LEF-1 gene was described. The amplified fragment is 1132bp (HM059927) in length, including an ORF of 1116 bp corresponding to a polypeptide of 371 amino acids residues with 41.2 kDa predicted molecular mass. The full cDNA nucleotide sequence has a 98% identity with bovine and 94% identity with transcript variant 2 of human. The amino acid sequence is 98% identical to the human isoform 2. Furthermore, a representative HMG-box and a β-catenin binding domain were predicted via the NCBI CDD (Conserved Domain Database). The goat LEF-1 gene cDNA was cloned and its molecular characterization was analyzed by bioinformatics. To our knowledge this is the first reported.
机译：LEF-1是淋巴细胞增强剂结合因子1，是高迁移率族（HMG）转录因子LEF-1 / TCF（T细胞特异性转录因子）家族的成员。 LEF-1 / TCF在Wnt /β-catenin信号通路的下游。 LEF-1需要Wnt信号传导和稳定的β-连环蛋白来表达头发特异性角蛋白基因并控制头发分化。 Tcf3 / LEF-1复合物在控制皮肤干细胞分化中起关键作用。因此，本研究通过RTPCR从内蒙古羊绒山羊（Capra hircus）扩增了LEF-1 cDNA，以生产出具有增加的羊绒产量的转基因山羊。描述了LEF-1基因的分子特征。扩增的片段长度为1132bp（HM059927），包括1116 bp的ORF，对应于具有41.2 kDa预测分子量的371个氨基酸残基的多肽。完整的cDNA核苷酸序列与人的牛具有98％的同一性，与人的转录本变体2具有94％的同一性。氨基酸序列与人同工型2具有98％的同一性。此外，通过NCBI CDD（保守域数据库）预测了具有代表性的HMG-box和β-catenin结合域。克隆了山羊LEF-1基因cDNA，并通过生物信息学分析了其分子特征。据我们所知，这是第一个报道。

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