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首页> 外文会议>Πανελληνιο Συνεδριο ΤροΦιμων >STUDY ON THE EFFECT OF SODIUM LACTATE, EDTA AND LACTOBACILLUS CURVATUS SOLUTIONS AGAINST SPOILAGE AND PATHOGENIC MICROORGANISMS ON PORK ESCALOPES UNDER MODIFIED ATMOSPHERE PACKAGING
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STUDY ON THE EFFECT OF SODIUM LACTATE, EDTA AND LACTOBACILLUS CURVATUS SOLUTIONS AGAINST SPOILAGE AND PATHOGENIC MICROORGANISMS ON PORK ESCALOPES UNDER MODIFIED ATMOSPHERE PACKAGING

机译：改性大气包装下乳酸钠，乙二胺四乙酸和弯孢乳杆菌溶液对细菌蒸发和致病微生物的影响

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The aim of this work was to improve the hygiene and shelf-life of pork escalopes preserved in MAP, by using mild hurdles and protective cultures(single/combined),which are not chemical preservatives and do not alter organoleptic characteristics of raw meat. Experimental design was as follows: Solutions M to S were adsorbed into pork escalopes(lOOg),using a vacuum tumbler, where M:Distilled water W:Solution of 0.9%NaCl L:Dispersion of Lb.curvatus+0.9%NaCl G:Solution of 5% sodium lactate+0.9%NaCl Evolution of 0.5%EDTA+0.9%NaCl LG:Dispersion of Lb.curvatus +5% sodium lactate+0.9%NaCl LE:Dispersion of Lb.curvatus +0.5%EDTA+0.9%NaCl GE:Solution of 5% sodium lactate+0.5%EDTA+0.9%NaCl LGE:Dispersion of Lb.curvatus +5% sodium lactate+0.5%EDTA+0.9%NaCl S:Supernatant of Lb.curvatus fermentation broth(containing curvacin) After the solutions were adsorbed, samples were packed in MAP(80%N2,20%CO2), and preserved at 4°C(x)22d(days).Sensory and microbiological analysis took place at 1,4,9,15,22d for the spoilage organisms: Pseudomonas, Brochothrix thermosphacta, Enterobacteriacae, Enterococcus, Yeasts. Also, the following pathogens were used for estimating the inhibition zones (well-diffusion assay) and the Minimum Inhibitory and Bacteriocidal Concentration (MIC/MBC): Escherichia coli. Salmonella typhimurium, Listeria monocytogenes. Bacillus cereus, Clostridium perfingens, Staphylococcus aureus, Campylobacter jejuni. These pathogens were also inoculated (～4log cfu/gr) in escalopes previously treated with solutions M,W,L,G,E. These samples were also packed in MAP, preserved at 4°C(x)20d,and pathogen populations were enumerated at 1d,5d,10d,15d. The results showed that spoilage organisms grew well until 15d, then became stable or declined. Single/combined treatments lead to significant reductions (up to 6log,at 15d or 22d). The inhibitory effect was clearest at 8d/9d,as the increase in microbial populations after 12d restricted the effectiveness of the solutions. At 22d all samples were organoleptically unacceptable. Enterobacteriacae dropped by 6log at 22d by adding LGE solution. Also, GE and S solutions were effective in reducing their population. Maximum antimicrobial activity against Brochothrix was achieved with solution S (5,5log reduction at 22d),and solution L. Most effective combination against Enterococci was solution LE. Pseudomonas spp. were in high numbers, despite the presence of CO_2,and reduced by 5log(15d) using solution S. Yeasts were less affected by any solution. Maximum anti-yeast activity occurred with solution GE. As for the pathogens, L.monocytogenes was significantly restricted by L.curvatus cells or curvacin. Similar inhibition was observed for B.cereus and C.perfingens. S.typhimurium and E.coli declined mostly due to lactate and EDTA. C.jejuni was significantly inhibited, not only by lactate and EDTA, but also by L curvatus, which also showed maximal inhibitory effect against S. aureus. The reduction in pathogens populations varied (1-4log reduction),depending on the microorganism and storage day. Overall, the activity of these solutions against L.monocytogenes and C.perfringens was important for ensuring the safety of these products, which may favor the growth of such pathogens. Shelf-life increased by 2-5 days, depending on the solution used, with LGE showing the best performance as concerns organoleptic and microbial characteristics. The application of Lcurvatus or curvacin,were useful in improving safety and shelf-life of escalopes.
机译：这项工作的目的是通过使用温和的，不是化学防腐剂且不会改变生肉感官特性的障碍物和保护性培养物（单一/混合），来改善MAP中保存的猪肉饭的卫生性和保质期。实验设计如下：使用真空转鼓将溶液M到S吸附到猪排（100 g）中，其中M：蒸馏水W：0.9％NaCl的溶液L：Lb.curvatus的分散液+ 0.9％NaCl的G：溶液乳酸钠5％+ 0.9％NaCl的演变0.5％EDTA + 0.9％NaCl LG的分布：弯曲乳杆菌+ 5％乳酸钠+ 0.9％NaCl的LE分散：弯曲乳杆菌+ 0.5％EDTA + 0.9％NaCl的GE ：5％乳酸钠+ 0.5％EDTA + 0.9％NaCl LGE的溶液：曲霉菌的分散液+ 5％乳酸钠+ 0.5％EDTA + 0.9％NaCl S：曲霉菌发酵肉汤（含姜黄素）的上清液溶液被吸附，样品装入MAP（80％N2,20％CO2）中，并保存在4°C（x）22d（天）。感官和微生物学分析分别在1,4,9,15,22d进行腐败菌：假单胞菌，嗜热溴化拟杆菌，肠杆菌，肠球菌，酵母。另外，以下病原体被用于估计抑制区（扩散分析）和最小抑制和杀菌浓度（MIC / MBC）：大肠杆菌。鼠伤寒沙门氏菌，李斯特菌。蜡状芽孢杆菌，产气荚膜梭菌，金黄色葡萄球菌，空肠弯曲菌。这些病原体也接种在事先用溶液M，W，L，G，E处理过的盲肠中（〜4log cfu / gr）。这些样品也装在MAP中，保存在4°C（x）20d，在1d，5d，10d，15d计数病原体种群。结果表明，腐败菌生长良好直至15d，然后趋于稳定或下降。单一/联合治疗导致显着减少（在15天或22天时高达6log）。抑制作用在8d / 9d最明显，因为12d后微生物种群的增加限制了溶液的有效性。在第22天，所有样品在感官上都不可接受。通过添加LGE溶液，肠杆菌在22天下降了6log。同样，GE和S解决方案在减少人口方面也很有效。用溶液S（在22d时降低5.5 log）和溶液L可获得对Brochothrix的最大抗菌活性。对肠球菌最有效的组合是溶液LE。假单胞菌尽管有CO_2的存在，但它们的数量还是很高的，并且使用溶液S减少了5log（15d）。酵母不受任何溶液的影响也较小。 GE溶液具有最大的抗酵母菌活性。至于病原体，单核细胞增生李斯特菌受到曲霉菌细胞或curvacin的显着限制。对于蜡状芽孢杆菌和穿孔芽孢杆菌也观察到类似的抑制作用。鼠伤寒沙门氏菌和大肠杆菌的下降主要归因于乳酸和EDTA。空肠弯曲杆菌不仅受到乳酸和EDTA的显着抑制，而且还受到弯曲曲霉的显着抑制，这也显示出对金黄色葡萄球菌的最大抑制作用。病原体种群的减少量有所不同（减少了1-4log），具体取决于微生物和保存日期。总体而言，这些溶液对单核细胞增生李斯特氏菌和产气荚膜梭状芽孢杆菌的活性对于确保这些产品的安全至关重要，这可能有利于此类病原体的生长。根据所用溶液的不同，保质期会增加2-5天，其中LGE在感官和微生物特性方面表现出最好的性能。 Lcurvatus或curvacin的应用对改善安全性和保鲜期很有用。

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