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Optimization of Biodemulsifier production condition in order to crude oil demulsification

机译：生物破乳剂生产条件的优化以原油破乳

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Paenibacillus alvei ARN63 as a biodemulsifier-producing bacterium has represented incredibledemulsifying ability. The produced lipopeptide biodemulsifier can be used in crude oil industryas an environmentally friendly (green) substance, containing no toxic component. Due toincrease production of biodemulsifier, as a type of biosurfactant, the culture conditions for thestrain were optimized. For this goal, the effect of different carbon sources and C/N ratios (carbonconcentration) were examined in detail at constant temperature, pH, shaking speed (rpm) andnitrogen source. Biodemulsifier synthesis and cell growth were determined via measurement ofsurface tension and optical density (OD600nm), respectively. Subsequently, to investigate itsdemulsification performance, the bottle test was carried out. Normal paraffin compounds such asC_(10)H_(22) and C_(12)H_(26) and motor oil favored the synthesis of this biodemulsifier. The best resultswere obtained employing motor oil as carbon source with a concentration of 50 ml/l. Thebiosurfactant (biodemulsifier) production was probably growth associated as resulted from thebiosurfactant production and growth kinetics. The surface tension was reduced (from 58 mN/m)to 24.7 mN/m in 72 h, and demulsification ratio reached to approximately 73%. Under optimalconditions, the biodemulsifier yield approached 2.1 g/l.
机译：作为生物破乳剂生产菌的肺炎Paenibacillus Alvei ARN63代表了令人难以置信的表现 破乳能力。生产的脂肽生物破乳剂可用于原油工业 作为一种环保（绿色）物质，不含任何有毒成分。由于 作为一种生物表面活性剂，增加生物破乳剂的生产， 菌株进行了优化。为了这个目标，不同碳源和碳/氮比（碳 在恒温，pH，摇动速度（rpm）和 氮源。生物破乳剂的合成和细胞生长是通过测量 表面张力和光密度（OD600nm）分别。随后，对其进行调查 破乳性能，进行了瓶试验。正构石蜡化合物，例如 C_（10）H_（22）和C_（12）H_（26）和机油促进了这种生物破乳剂的合成。最好的结果 用机油作为碳源以50 ml / l的浓度获得汽油。这 生物表面活性剂（生物破乳剂）的生产可能与增长有关，这是由于 生物表面活性剂的产生和生长动力学。表面张力降低（从58 mN / m起） 在72小时内达到24.7 mN / m，破乳率达到约73％。在最佳状态 在此条件下，生物破乳剂的产量接近2.1 g / l。

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