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Optimization of Biodemulsifier production condition in order to crude oil demulsification

机译:优化生物乳化剂生产条件,以原油破乳

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Paenibacillus alvei ARN63 as a biodemulsifier-producing bacterium has represented incredible demulsifying ability. The produced lipopeptide biodemulsifier can be used in crude oil industry as an environmentally friendly (green) substance, containing no toxic component. Due to increase production of biodemulsifier, as a type of biosurfactant, the culture conditions for the strain were optimized. For this goal, the effect of different carbon sources and C/N ratios (carbon concentration) were examined in detail at constant temperature, pH, shaking speed (rpm) and nitrogen source. Biodemulsifier synthesis and cell growth were determined via measurement of surface tension and optical density (OD600nm), respectively. Subsequently, to investigate its demulsification performance, the bottle test was carried out. Normal paraffin compounds such as C10H22 and C12H26 and motor oil favored the synthesis of this biodemulsifier. The best results were obtained employing motor oil as carbon source with a concentration of 50 ml/l. The biosurfactant (biodemulsifier) production was probably growth associated as resulted from the biosurfactant production and growth kinetics. The surface tension was reduced (from 58 mN/m) to 24.7 mN/m in 72 h, and demulsification ratio reached to approximately 73%. Under optimal conditions, the biodemulsifier yield approached 2.1 g/l.
机译:Paenibacillus Alvei Arn63作为生物乳化剂产生的细菌具有令人难以置信的破乳能力。产生的脂肽生物乳剂可用于原油工业,作为环保(绿色)物质,含有无毒成分。由于生物乳化剂的产量增加,作为一种生物表面活性剂,优化菌株的培养条件。为此目的,在恒温,pH,摇动速度(RPM)和氮源处详细检查不同碳源和C / N比(碳浓度)的效果。通过测量表面张力和光密度(OD600nm)测定生物乳膏合成和细胞生长。随后,为了研究其破乳性能,进行瓶试验。正常的石蜡如C10H22和C12H26和机油赞成该生物乳化剂的合成。获得的最佳结果是使用电动油作为碳源,浓度为50ml / l。由于生物活性剂的生产和生长动力学产生,生物活性剂(生物乳化剂)生产可能产生的生长。将表面张力降低(从58mN / m),在72小时内降低至24.7mN / m,并且破乳率达到约73%。在最佳条件下,生物乳化剂收益率接近2.1克/升。

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