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首页> 外文会议>World Congress on Medical Physics and Biomedical Engineering >How Does Cell Deform Through Micro Slit Made by Photolithography Technique?
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How Does Cell Deform Through Micro Slit Made by Photolithography Technique?

机译：电池如何通过光刻技术制成的微狭缝变形？

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Several slits sort cell according to the deformability in vivo. A micro slit (0.87 mm of width, 0.010 mm height) was newly designed between a micro ridge on a transparent polydimethylsiloxane plate and micro ridges on a borosilicate glass plate. These ridges made by photolithography technique make contact each other in the perpendicular position to make the slit between the ridges. A one-way flow system was designed to observe each cell passing through the slit in vitro. Four kinds of cells were used in the test: C2C12 (mouse myoblast cell), HUVEC (human umbilical vein endothelial cell), Hepal-6 (mouse hepatoma cell), and Neuro-2a (mouse neural crest-derived cell). The suspension of each kind of cells was injected to the slits. The deformation of each cell passing through the slit was observed with an inverted phase-contrast microscope. At the microscopic images, the outline of each cell was traced, and the area (S) was calculated. The deformation ratio was calculated as the ratio (S2/S1) of the projected area of each cell before the slit (S1) and that in the slit (S2). The velocity of the cell passing through the slit was calculated by the trace at the microscopic movie. The experimental results show that each cell deforms to the flat circular disk and passes through the micro slit. Hepal-6 is flattened with the increase of the passing velocity, and HUVEC is elongated along the flow. The designed slit between micro ridges is effective to evaluate the deformability of cells.
机译：几个狭缝根据体内变形性进行分拣电池。在透明聚二甲基硅氧烷板上的微脊和硼硅酸盐玻璃板上的微脊之间新设计微狭缝（宽度0.87mm宽度，0.010mm高度）。通过光刻技术制造的这些脊在垂直位置彼此接触以使脊之间的狭缝。单向流动系统被设计成观察通过体外穿过狭缝的每个细胞。在试验中使用四种细胞：C2C12（小鼠肌细胞），HUVEC（人脐静脉内皮细胞），肝-6（小鼠肝癌细胞）和神经2A（小鼠神经嵴衍生细胞）。将每种细胞的悬浮液注入狭缝。用倒置相位对比显微镜观察通过狭缝的每个细胞的变形。在微观图像中，追踪每个电池的轮廓，并计算区域。变形比计算为狭缝（S1）之前每个单元的投影区域的比率（S2 / S1）（S1），在狭缝中（S2）。通过狭缝的细胞的速度通过微透视电影的痕量计算。实验结果表明，每个细胞变形到扁平圆盘并通过微狭缝。随着通过速度的增加，Hepal-6呈平坦化，Huvec沿流动延长。微脊之间的设计狭缝有效地评估细胞的可变形性。

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