• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International symposium on bioanalytical chemistry >PURIFICATION OF A CLONED ENZYME-FUNGI NITRIC OXIDE REDUCTASE (FUSARIUM OXYSPORUM P-450 NOR)
【24h】

PURIFICATION OF A CLONED ENZYME-FUNGI NITRIC OXIDE REDUCTASE (FUSARIUM OXYSPORUM P-450 NOR)

机译：纯化克隆酶 - 真菌一氧化氮还原酶（牡蛎P-450也不是）

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Denitrification activities were considered to be characteristic of prokaryotes, but in resent years it was found that several fungi could form nitrous oxide(N_2O)from nitrate or nitrite. A unique cytochrome P-450 is involved in fungal denitrification, acting as a nitric oxide reductase (P - 450nor). P-450nor shows several interesting properties. It catalyzes the reduction of NO to N_2O in the presence of NADH wthout mediation by any other proteinaceous components ,and seems to receive electrons directly from NADH. We successfully cloned Fungus (Fusarium oxysporum)P - 450nor cDNA, and expressed in quantity by a expression vector-pCW in E. coli. The cloned enzyme-F. P-450nor was purified with DEAE -cellulose and FPLC system.
机译：反硝化活性被认为是原核生物的特征，但在怨恨年份中发现，几个真菌可以从硝酸盐或亚硝酸盐形成氧化二氮（N_2O）。独特的细胞色素P-450涉及真菌脱氮，作用作一氧化氮还原酶（P - 450nor）。 P-450nor显示了几个有趣的属性。它催化在NADH WHOUT中调解的情况下通过任何其他蛋白质组分的存在，并且似乎直接从NADH接收电子。我们成功地克隆了真菌（牡蛎）p-450nor cDNA，并通过大肠杆菌中的表达载体-CCW表达数量。克隆的酶-f。用DEAE - 纤维素和FPLC系统纯化P-450nor。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号