How to Process a Dilute Ejaculate of Semen for Cooled-Transported Insemination

机译：如何处理精液稀释的精液进行冷却运输的授精

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To optimize preservation of spermatozoa for cooled-transported insemination, dilute ejaculates of semen containing <100 million spermatozoa/ml are generally centrifuged in order to concentrate the spermatozoa and remove some of the seminal plasma. Centrifugation is performed by mixing raw semen 1:1 with extender and dispensing the extended semen in 40-ml aliquots and spinning for 10 to 12 minutes at a centrifugal force of 300 to 400 times gravity. This results in the recovery of approximately 75% ofthe spermatozoa in each tube. After centrifugation, 30 ml of the supernatant is removed and 20 ml fresh extender is added to each tube and the pellet is resuspended, which empirically produces a final concentration of spermatozoa approximately one-half the initial concentration (in the raw semen) in a mixture containing 17% seminal plasma.

机译：为了优化用于冷却输送的授精的精子的保存，含有<×100百万种精子/ ml的精液的稀释射精以浓缩精子并除去一些精子等离子体。通过将粗糙的精液1：1用增量剂混合并在40ml等分试样中将延长的精液分配并在300至400次重力的离心力下旋转10至12分钟来进行离心。这导致在每个管中恢复约75％的精子。离心后，除去30ml上清液，向每个管中加入20ml新鲜的延长剂，重新悬浮沉淀，颗粒在混合物中致致粒子的最终浓度大约一半的初始浓度（在未经原子中的精液中）含有17％的精血浆。

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来源
《Annual Convention of the American Association of Equine Practitioners》|2008年||共5页
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Dirk K. Vanderwall;
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中图分类 S821-532;
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2. Functional characterisation of semen in honeybee queen ( A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination [J] . Marco Lodesani, Donatella Balduzzi, Andrea Galli Italian journal of animal science . 2004,第4期

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4. How to Process a Dilute Ejaculate of Semen for Cooled-Transported Insemination [C] . Dirk K. Vanderwall Annual Convention of the American Association of Equine Practitioners . 2008

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How to Process a Dilute Ejaculate of Semen for Cooled-Transported Insemination

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