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Evolutionary and molecular genetic studies at the waxy locus in cultivated rice and wild relatives

机译:栽培稻和野生亲属蜡质基因座的进化和分子遗传学研究

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We investigated polymorphism in the waxy Wx locus among wild and cultivated rice. p-SINEl-r2, a retrotransposon inserted at the Wx locus in Oryza sativa, showed polymorphism between wild rice 0. nivara and 0. rufipogon. This p-SINEl polymorphism couldexplain 70% of the annual-perennial differentiation, and it also corresponds to indiea-japonica differentiation in wild relatives. We also analyzed the first intron-exon splice site (AG/GT or AG/TT; G-T polymorphism). It was suggested that G-T polymorphism corresponds to Wx~a-Wx~b and indiea-japonica, respectively. Wild relatives possessed an AG/GT type without exception. In 300 waxy strains, they have a mostly AG/TT (japoniea-type) sequence, except for 10 strains, independently of indiea-japonica differentiation. It was concluded that waxy mutation and waxy rice cultivation had originated from japonica.
机译:我们在野生和栽培水稻中调查了蜡质WX基因座中的多态性。 P-Sinel-R2,插入在WX位点的RECRORANSPOSON在ORYZA Sativa中,在野生米0. nivara和0. Rufipogon之间显示多态性。这种p-sinel多态性同意年度多年生分化的70%,它还对应于野生亲属的靛蓝粳稻分化。我们还分析了第一个内部内外剪接部位(Ag / gt或Ag / tt; G-T多态性)。有人建议,G-T多态性分别对应于WX〜A-Wx〜B和靛蓝。野生亲属具有AG / GT型而无需异常。在300蜡质菌株中,除了10株的抗原,除了靛蓝 - 粳稻分化外,它们具有大部分Ag / tt(japoniea型)序列。结论是,蜡质突变和糯米栽培源于粳稻。

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