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首页> 外文会议>Annual Meeting of the Plant Growth Regulation Society of America >EMBRYOGENESIS INDUCTION WITH IAA AND IAA CONJUGATES IN CARROTS
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EMBRYOGENESIS INDUCTION WITH IAA AND IAA CONJUGATES IN CARROTS

机译：胚胎发生与红萝卜中的IAA和IAA缀合物诱导

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摘要

In order for cells to form embryos they must initially be exposed to auxins before being transferred into an auxin-free medium. Synthetic auxins, such as 2,4-dichlorophenoxyacetic acid (2,4-D), generate embryos with genetic variations and other negative side effects. Natural auxins, like indole-3-acetic acid (IAA), can provide an alternative way to induce somatic embryogenesis. Our research has shown that it is possible to use amide-conjugated IAA in place of 2,4-D to induce embryo formation. It maybe possible to use free IAA to accomplish the same feat. It has also been determined that ethanol-based stock solutions of IAA alter a culture's ability to form embryos, but water-based solutions of IAA do not. Water-based stock solutions of IAA are generated using the potassium salt of IAA, which does not substantially change the pH of culture medium. Cellular metabolism of IAA has been determined by examining the concentrations of IAA and its conjugates in cells and culture media that have been treated with IAA over a period of 24 hours. Based on preliminary data using ethanol-based IAA solutions, it would be necessary to replenish IAA daily to ensure that it acts as an effective auxin.
机译：为了形成细胞形成胚胎，它们必须最初暴露于转移到无毒培养基中之前暴露于毒素。合成菌素，例如2,4-二氯苯乙烯酸（2,4-D），产生具有遗传变异和其他负副作用的胚胎。像吲哚-3-乙酸（IAA）一样的天然毒素可以提供诱导体细胞胚胎发生的替代方法。我们的研究表明，可以使用酰胺缀合的IAA代替2,4-D以诱导胚胎形成。可以使用免费的IAA来完成同样的壮举。还确定了IAA的基于乙醇的股票溶液改变了培养的形成胚胎的能力，而是IAA的水性溶液不会。使用IAA的钾盐产生IAA的水基储备溶液，其不会大大改变培养基的pH。通过检查在24小时内用IAA治疗的细胞和培养基中的IAA和其缀合物的浓度和其缀合物的浓度确定IAA的细胞代谢。基于使用基于乙醇的IAA解决方案的初步数据，必须每天补充IAA，以确保其作为一种有效的养肝剂。

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