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首页> 外文会议>International Symposium on the Biosafety of Genetically Modified Organisms >Monitoring the identity and survival of genetically modified or non-modified plant growth promoting bacteria and their impact on soil microbial communities
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Monitoring the identity and survival of genetically modified or non-modified plant growth promoting bacteria and their impact on soil microbial communities

机译：监测遗传修饰或未改性植物生长促进细菌的身份和存活及其对土壤微生物社区的影响

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Recent research on rhizosphere microbial communities by means of advanced molecular tools has clearly shown that the composition of microbial communities in rhizosphere soils is highly dynamic. The relative abundance of microbial populations in the rhizosphere was shown to be dependent on the plant species and the soil. The improved understanding of microbe-plant-environment interactions will improve future applications of PGPR strains. The tool set available to characterise the identity of microbial inoculants has been considerably improved making it possible to regularly confirm strain identity and the stability of the genetic modification. In particular for genetically modified strains, specific and sensitive methods to track the inoculant strain in the environment are available, either based on PCR amplification with construct specific primers or by means of reporter gene products. However, methods to follow the expression of genes, e.g. involved in biocontrol, are still in their infancy. While molecular fingerprinting techniques either based on the analysis of 16S or 18S rDNA amplified from directly extracted DNA allow to rapidly determine potential effects of microbial inoculants on the structural composition of microbial communities, monitoring methods which determine the potential impacts of inoculants on the soil functioning are not yet fully available. While monitoring the fate of microbial inoculants and their potential impacts on soil microbial communities should be analysed in the phase of field testing, the regular testing of inoculants strain identity is particularly important when the strains are used in a larger scale. Thus capacity building in countries which use PGPR strains on a large scale is an important prerequisite for the safe and successful use of microbial inoculants.
机译：最近通过先进的分子工具对根际微生物群落的研究已经清楚地表明，根际土壤中微生物社区的组成具有高度动态的。根际的微生物群体的相对丰富依赖于植物物种和土壤。改善对微生物 - 植物环境相互作用的理解将改善PGPR菌株的未来应用。可用于表征微生物接种剂的身份的工具集已经显着改善，使得可以定期确认遗传修饰的应变同一性和稳定性。特别是对于追踪环境中的粉碎菌株的特异性和敏感的方法可用于基于与构建特异性引物的PCR扩增或通过报告基因产物进行PCR扩增。但是，遵循基因表达的方法，例如，涉及生物控制，仍在他们的婴儿期间。而基于从直接提取的DNA扩增的16S或18S RDNA分析的分子指纹识别技术允许快速确定微生物孕育剂对微生物群体结构组成的潜在影响，但确定接种剂对土壤功能潜在影响的监测方法是尚未完全可用。在监测微生物孕育剂的命运及其对土壤微生物社区的潜在影响的同时，应在现场测试的阶段分析，当菌株以较大规模使用时，孕制剂的定期测试尤为重要。因此，在大规模上使用PGPR菌株的国家的能力建设是安全和成功使用微生物接种剂的重要前提。

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