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首页> 外文会议>International Congress on Nitrogen Fixation >Map based cloning of a receptor kinase gene (Nork) by genetic mapping of a mutation (nn_1) conditioning non-nodulating phenotype in the tetraploid alfalfa mutant MN-1008
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Map based cloning of a receptor kinase gene (Nork) by genetic mapping of a mutation (nn_1) conditioning non-nodulating phenotype in the tetraploid alfalfa mutant MN-1008

机译：通过突变（NN_1）调节在四倍体Alfalfa突变体MN-1008中的突变映射（NN_1）条件的遗传映射来克隆受体激酶基因（Nork）的克隆

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MN-1008 is a non-nodulating (Nod~-) mutant of alfalfa (Medicago saliva L.) isolated and described by Peterson and Barnes (1981). The mutant plants showed neither root hair deformation, cortical cell division nor calcium spiking after inoculation with Sinorhizobium meliloti or Nod factor (NF) treatment (Dudley, Long 1989; Ehrhardt et al. 1996; Endre et al, 1996). Variation in membrane depolarization activity was demonstrated by Felle et al. (1996) using individual plantlets from the MN-1008 seed population. In addition MN-1008 was resistant not only to Rhizobium infection but to vesicular-arbuscular mycorrhiza colonization as well (Brudbury et al. 1991). Spontaneous nodulation in the absence of Rhizobium (referred to as Nar phenotype, Truchet et al. 1989) is one of the unique features of alfalfa. Interestingly the non-nodulation alfalfa mutant, MN-1008, did not lose this character and was susceptible to spontaneous nodulation (Caetano-Annoles et al. 1993). In accordance with this phenomenon earlynodulins (e.g. ENOD2 and ENOD40) could be induced by cytokinin (Hirsch et al 1997) implying that in this mutant the extrinsic signal perception was abolished but the capacity of nodule initiation and development, as inner genetic program of the plant, was intact. As a consequence, the identification and isolation of the gene conditioning the non-nodulation phenotype in mutant MN1008 would shed light on a key function involved hi the initiation of the signal transduction pathway leading to nodule formation. To achieve this goal the map-based cloning strategy was adapted for the tetraploid alfalfa.
机译：Mn-1008是苜蓿（Medicago Saliva L.）的非旋结（NOD〜）突变体，由Peterson和Barnes（1981）描述。突变植物既不显示根毛发变形，皮质细胞划分也没有用Sinorhizobium Meliloti或Nod因子（NOD）治疗（Dudley，Long 1989; Ehrhardt等人1996; Endre等，1996）。 Felle等人证明了膜去极化活性的变化。 （1996）使用来自MN-1008种子种群的个体种植体。此外，Mn-1008不仅耐根序感染，而且对胚胎骨髓菌疹殖民化也有抗性（Brudbury等，1991）。在没有根茎的情况下（称为NAR表型，Truchet等，1989）是苜蓿的独特特征之一。有趣的是，非染色苜蓿突变体Mn-1008并未丧失这种性格，并且易患自发染色的（Caetano-Annoles等，1993）。根据这种现象，可以通过Cytokinin（HIRSCH等人1997）诱导这种现象（例如ENOD2和ENOD40），这意味着在该突变体中，外部信号感知被废除，但结节引发和发育的能力是植物的内遗传程序，完好无损。因此，基因调节基因调节的鉴定和分离突变体MN1008中的非染色表型将在关键函数上脱光，涉及引起结节形成的信号转导途径的启动。为了实现这一目标，将基于地图的克隆策略适用于四倍体苜蓿。

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