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Electrical impedance spectroscopy for on-line estimation of viable biomass

机译：用于可行生物质的在线估计的电气阻抗光谱

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Electrical Impedance Spectroscopy (EIS) consists on the measurement of electrical impedance at different frequencies. The electrical impedance of a biological sample has a specific behavior with respect to the frequency of the electrical current injected. This behavior can be distinguished from that of a sample without cells or with dead cells. NTE started the development of this technology in the frame of a project of the European Space Agency (1) and in collaboration with the UPC. This technology has started a technology transfer process, in which three companies from three different fields: a brewery (Freixenet, S.A.), a water treatment plant engineering company (Indeco, S.A.) and a pharmaceutical company (Biokit, S.A.) have evaluated the technology for their production requirements. Specifications were issued for each process considered in case: -On-line viable yeast cell determination in CAVA production line. -Determination of viable biomass in activated sludge in the biological reactor of a water treatment plant. -Determination of Eschericia coli concentration in a protein production process. NTE designed a system for each case with specific electrodes, electronics and software. These systems were tested at each company in each industrial process. Requirements on Biomass determination in threshold, resolution, repeatability and accuracy were set. The tests have shown compliance of the systems with the requirements. The following results were obtained for each application: -On-line viable yeast cell determination. The technology is able to measure on-line the amount of viable yeast in the fermentation tanks and monitor the whole growth process. -Determination of viable biomass in activated sludge. The technology is able to provide the biomass concentration from low concentration values and to give an estimation on the distribution of the population in the activated sludge (from bacterial to algae) Determination of Eschericia coli concentration. The technology is able to monitor on-line the viable biomass during growth process in a bioreactor.
机译：电阻抗光谱（EIS）包括在不同频率下测量电阻抗。生物样品的电阻抗相对于注入的电流的频率具有特定的行为。这种行为可以与没有细胞或死细胞的样本的这种行为区分开。 NTE开始在欧洲航天局（1）项目的框架中发展这项技术，并与UPC合作。该技术已启动技术转让过程，其中三家不同的三个不同领域：啤酒厂（Freixenet，SA），水处理厂工程公司（Indeco，SA）和制药公司（Biokit，SA）已经评估了该技术为他们的生产要求。在案例中审议的每种过程发出规范：CAVA生产线中的碱水可行酵母细胞测定。 - 水处理厂生物反应器中活性污泥中活性污泥的活性生物量。 - 在蛋白质生产过程中进行大肠杆菌浓度的测定。 NTE设计了一种具有特定电极，电子和软件的每种情况的系统。这些系统在每个工业过程中的每家公司进行了测试。设定了对阈值，分辨率，可重复性和准确度的生物质测定的要求。该测试已显示系统的符合要求。为每种应用获得以下结果：-ON-LINE可行的酵母细胞测定。该技术能够在发酵罐中测量可行酵母的数量，并监测整个生长过程。 - 在活性污泥中的可行生物质进行测定。该技术能够从低浓度值提供生物质浓度，并估计活性污泥（从细菌到藻类）中群体的分布估计，从而测定大肠杆菌浓度。该技术能够在生物反应器中的生长过程中在线监测可行的生物质。

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