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首页> 外文会议>Annual Rocky Mountain Bioengineering Symposium >GROWTH AND CELL VIABILITY OF ESTRADIOL AND IP-6 TREATED HEP-2 LARYNGEAL CARCINOMA CELLS
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GROWTH AND CELL VIABILITY OF ESTRADIOL AND IP-6 TREATED HEP-2 LARYNGEAL CARCINOMA CELLS

机译：雌二醇和IP-6治疗HEP-2喉癌细胞的生长和细胞活力

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Inositol 6-phosphate (IP-6) has demonstrated novel anti-cancer activity using several different tumor models. IP-6, a phytoestrogen, has estrogen receptor (ER) binding capabilities that are not known to cause cellular proliferation in hormone sensitive cells. It is hypothesized that IP6 can induce competitive inhibition with estrogen for estrogenic binding sites on cancer cells resulting in decreased proliferation. In this experiment, Hep-2 cells were treated with Estradjol and IP-6 in a dose dependant manner for 24, 48, and 72 hours. They were analyzed for changes in number, protein concentrations, damage, and morphology. There was an increase in cell proliferation in Estradiol treated Hep-2 cells. Cells treated with IP-6 showed no change in cell proliferation in the 24 or 48-hour groups, but there was a decrease in number with the 72-hour group, particularly with the 1mM dose. Both the Estradiol and P-6 treatments caused no membrane oxidation and the level of protein synthesis stayed consistent. The morphology showed small round to cuboidal, single cells with scant, dense, basophilic cytoplasm and hyperchromatic nuclei with smooth borders. Some cells showed anucleation and cellular degeneration. Although IP-6 is a phytoestrogen, the results show that affinity for estrogen binding sites on Hep-2 cells is greatly decreased. However, with time given increased concentrations, IP-6 can cause a decrease in the cellular proliferation of Hep-2 cells without initiating cellular apoptosis.
机译：肌醇6-磷酸盐（IP-6）已经证明了使用几种不同肿瘤模型的新型抗癌活性。 IP-6，一种植物雌激素，具有雌激素受体（ER）结合能力，所述雌激素敏感性敏感细胞中的细胞增殖。假设IP6可以诱导竞争性抑制雌激素在癌细胞上的雌激素结合位点导致增殖降低。在该实验中，HEP-2细胞以雌二醇和IP-6处理，剂量依赖性方式24,48和72小时。分析它们的数量，蛋白质浓度，损伤和形态学的变化。雌二醇治疗HEP-2细胞中细胞增殖增加。用IP-6处理的细胞显示24或48小时组中细胞增殖的变化，但是数量减少，72小时组，特别是1mm剂量。雌二醇和P-6治疗均导致膜氧化，蛋白质合成水平保持一致。形态学表现出小圆形的单细胞，单细胞，具有瘢痕，致密，嗜碱性细胞质和高核核，具有平滑的边界。一些细胞显示出肌肉和细胞变性。尽管IP-6是一种植物雌激素，但结果表明，对Hep-2细胞的雌激素结合位点的亲和力大大降低。然而，随着时间的推移，随着时间的推移，IP-6可导致HEP-2细胞的细胞增殖降低而不引发细胞凋亡。

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