A Novel Microinjection System by Fusing Glass Needle and PDMS-Based Valve with Rigid Center Membrane

机译：具有刚性中心膜的玻璃针和基于PDMS阀门的新型显微注射系统

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We developed a microinjection system by combining a pulled glass microneedle and a PDMS-based micro valve which can deliver subnano liter samples into a single oocyte. The volume of solution was regulated by a pneumatically operated valve. The estimated minimum droplet volume using manual regulation was 0.9 nl. Feasibility experiments to deliver the solution to the embryo were performed.

机译：我们通过组合拉动的玻璃微针和基于PDMS的微阀来开发了一种微注射系统，这可以将亚纳纳升样品递送到单个卵母细胞中。通过气动操作阀调节溶液的体积。使用手动调节的估计的最小液滴体积为0.9 nL。进行递送胚胎溶液的可行性实验。

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《International conference on miniaturized chemical and biochemical analysis systems》|2003年||共4页
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S. H. Lee; D. J. Beebe;
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中图分类生物化学技术;
关键词
microinjection system; PDMS; embryo; microvalve;

机译：显微注射系统;PDMS;胚胎;微型阀;

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1. Assembly and Simple Demonstration of a Micropump Installing PDMS-Based Thin Membranes as Flexible Micro Check Valves [J] . Yo Tanaka, Kae Sato, Takehiko Kitamori Journal of biomedical nanotechnology . 2009,第5期

机译：微型泵的组装和简单演示，该微型泵将基于PDMS的薄膜安装为柔性微型止回阀
2. Numerical analysis and characterization of bionic valves for microfluidic PDMS-based systems [J] . I. Klammer, A. Buchenauer, H. Fassbender, Journal of Micromechanics and Microengineering . 2007,第7期

机译：基于微流体PDMS的系统中仿生阀的数值分析和表征
3. Glass needle-mediated microinjection of macromolecules and transgenes into primary human mesenchymal stem cells. [J] . Tsulaia TV, Prokopishyn NL, Yao A, Journal of biomedical science. . 2003,第3期

机译：玻璃针介导的大分子和转基因显微注射到原代人间充质干细胞中。
4. A Novel Microinjection System by Fusing Glass Needle and PDMS-Based Valve with Rigid Center Membrane [C] . S. H. Lee, D. J. Beebe International conference on miniaturized chemical and biochemical analysis systems . 2003

机译：具有刚性中心膜的玻璃针和基于PDMS阀门的新型显微注射系统
5. Digital control of wheel loader implement system using closed center valve and variable displacement pump. [D] . Chen, Chenyao. 1999

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6. Bronchotracheal Stenting Management by Rigid Bronchoscopy under Extracorporeal Membrane Oxygenation (ECMO) Support: 10 Years of Experience in a Tertiary Center [O] . Sabrina Meyer, Anne-Sophie Dincq, Lionel Pirard, 2021

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7. Design and Fabrication of a PDMS-Based Manual Micro-Valve System for Microfluidic Applications [O] . Tu N. Le, Van-Anh Nguyen, Giang L. Bach, 2020

机译：基于PDMS的微流体应用的手动微阀系统的设计与制造

A Novel Microinjection System by Fusing Glass Needle and PDMS-Based Valve with Rigid Center Membrane

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