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首页> 外文会议>International Wheat Genetics Symposium >A genetic linkage map of durum x Triticum dicoccoides backcross population and QTL analysis for yellow pigment and protein content.
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A genetic linkage map of durum x Triticum dicoccoides backcross population and QTL analysis for yellow pigment and protein content.

机译：硬粒X triticum dicoccoides Rescross群体遗传联系地图和黄色颜料和蛋白质含量的QTL分析。

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High protein content and gluten strength in durum (Triticum turgidum L. var durum) are important to produce pasta; couscous; and burghul. The aim of this study was to construct a duram genetic linkage map and to determine QTLs linked to grain-quality. The studied OmrabiS/ Triticum dicoccoides 600545// Omrabi5 population comprises 114 RILs and the markers used are Single Sequence Repeats markers (SSRs); Amplified Fragment Length Polymorphism markers (AFLPs); and seed storage proteins (SSP). Yellowpigment (YP) and protein content (PC) were analyzed over 18 environments using Near-Infra-Red and chemical protocols. The map consisted of 14-dumm chromosomes plus an unknown group. Analysis of QTLs was based on simple and simplified composite intervalmapping (SIM and sCIM). PC was highly affected by environments. Six PC QTLs were detected on 4BL; 3BS; and 6AS explaining 27% of the total variation and showing QTLxE interaction effects; two of them were linked to vitreousness. The QTLs explanation rate was higher in stressed than in non-stressed environments. T. dicoccoides 600545 alleles improved significantly the PC content. For YP; 3 QTLs were detected on the telomeric regions of 7AL and 7BL; explaining 62% of the total variation. The detected QTLs will be used in the marker assisted breeding program.
机译：杜兰姆（Triticum Turgidum L. Var Durum）的高蛋白质含量和麸质强度是产生意大利面的重要性;库斯库斯;和Burghul。本研究的目的是构建DurAM遗传联系地图，并确定与籽粒质量相关的QTL。研究的omrabis / triticum dicoccoides 600545 // omrabi5群包含114个rils，使用的标记是单序重复标记（SSR）;扩增的片段长度多态性标记物（AFLP）;和种子储存蛋白（SSP）。使用近红外红色和化学方案分析了18个环境中的丝晶（YP）和蛋白质含量（PC）。地图由14-dummosomes加上一个未知组组成。 QTL的分析是基于简单简化的复合间隔（SIM和SCIM）。 PC受环境的高度影响。在4BL上检测到六个PC QTL; 3bs;和6AS解释总变化的27％，显示QTLXE互动效应;其中两个与玻璃体有关。 QTLS的解释率高于非压力环境。 T. Dicoccoides 600545等位基因显着改善了PC含量。 yp;在7AL和7BL的端粒区域上检测到3个QTL;解释总变异的62％。检测到的QTL将用于标记辅助繁殖计划。

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