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Chromosome analysis and sorting in durum wheat and physical mapping of repetitiveDNA sequences.

机译：染色体分析和分类在硬粒小麦和重复性序列的物理映射中。

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A protocol for preparation of suspension of intact chromosomes of duram wheat from synchronized root tips is described. Histograms of fluorescence intensity (flow karyotypes) obtained after the analysis of DAPI-stained chromosomes contain two composite peaks and no single chromosome may be discriminated. However, the use of double ditelosomic lines facilitates sorting of all chromosome arms. Using FISH on flow-sorted chromosomes, genomic distribution of four repetitive DNA sequences have been determined. An unexpected finding of this study is the discovery that 'Langdon' + 'Chinese Spring' telosomic hues may be used to sort any chromosome arm of hexaploid wheat. Thus, the tools are available to fractionate the genomes of wheat to simplify their analysis.

机译：描述了一种制备来自同步根尖端的DurAm小麦的完整染色体的悬浮液的方案。在DAPI染色染色体分析后获得的荧光强度（流核型）的直方图含有两个复合峰，并且可以区分单个染色体。然而，使用双Diteloomic系有助于所有染色体臂的分选。在流量排序的染色体上使用鱼类，已经确定了四种重复DNA序列的基因组分布。这项研究的意外发现是发现'兰登'+'中国春天'专题色调可用于对六倍体小麦的任何染色体臂进行分类。因此，该工具可用于分馏小麦的基因组以简化其分析。

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《International Wheat Genetics Symposium》|2003年||共3页
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M. Kubalakova; J. Bartos; P. Kovarova; J. Cihalikova; N. Watanabe; S. F. Kianian; J. Doleze;
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中图分类 S512.1-532;
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1. A consensus framework map of durum wheat (Triticum durum Desf.) suitable for linkage disequilibrium analysis and genome-wide association mapping [J] . Marco Maccaferri, Maria A Cane’, Maria C Sanguineti, BMC Genomics . 2014,第1期

机译：适用于连锁不平衡分析和全基因组关联映射的硬质小麦（Triticum durum Desf。）的共识框架图
2. Identification of Lens culinaris ssp. culinaris chromosomes by physical mapping of repetitive DNA sequences. [J] . Galasso I, Schmidt T, Pignone D Chromosome research: An international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology . 2001,第3期

机译：Lens culinaris ssp的鉴定。通过重复DNA序列的物理作图来绘制culinaris染色体。
3. Molecular mapping of a leaf rust resistance gene on the short arm of chromosome 6B of durum wheat. [J] . Herrera-Foessel S. A., Singh R. P., Huerta-Espino J., Plant Disease . 2008,第12期

机译：硬粒小麦6B号染色体短臂上的抗叶锈基因的分子作图。
4. Chromosome analysis and sorting in durum wheat and physical mapping of repetitiveDNA sequences. [C] . M. Kubalakova, J. Bartos, P. Kovarova, International Wheat Genetics Symposium . 2003

机译：染色体分析和分类在硬粒小麦和重复性序列的物理映射中。
5. Homoeologous Recombination-Based Chromosome Engineering for Physical Mapping and Introgression in Wheat and Its Relatives Aegilops speltoides and Thinopyrum elongatum [D] . Zhang, Mingyi. 2020

机译：小麦物理映射和血栓渗流的副主态重组染色体工程及其亲属蛇肌肉和薄孔龙
6. A consensus framework map of durum wheat (Triticum durum Desf.) suitable for linkage disequilibrium analysis and genome-wide association mapping [O] . Marco Maccaferri, Maria Angela Cane’, Maria C Sanguineti, 2014

机译：适用于连锁不平衡分析和全基因组关联映射的硬质小麦（Triticum durum Desf。）的共识框架图
7. A consensus framework map of durum wheat (Triticum durum Desf.) suitable for linkage disequilibrium analysis and genome-wide association mapping [O] . Maccaferri M., Cane M. A., Sanguineti M. C., 2014

机译：适用于连锁不平衡分析和全基因组关联映射的硬质小麦（Triticum durum Desf。）的共识框架图

Chromosome analysis and sorting in durum wheat and physical mapping of repetitiveDNA sequences.

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