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首页> 外文会议>Annual Rocky Mountain Bioengineering Symposium >CULTURED KERATINOCYTES AND DERMAL FIBROBLASTS ON A DOUBLE-LAYER SCAFFOLD WITH BI-MEDIUM CULTURE SYSTEM
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CULTURED KERATINOCYTES AND DERMAL FIBROBLASTS ON A DOUBLE-LAYER SCAFFOLD WITH BI-MEDIUM CULTURE SYSTEM

机译：用双层支架与双层支架培养的角质形成细胞和皮肤成纤维细胞与双培养基培养系统

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The increasing survival rate of massive burn patients, whose available donor sites for autografting are very limited, results in the emerging development and requirement of artificial skin [1-4]. The objective of this study is to produce cultured autologous keratinocyte grafts and to develop an optimal skin substitute for permanent wound closure. in the development of skin equivalent, human dermal fibroblasts were seeded to form three-dimensional dermal replacement tissue. The keratinocytes were initially cultured in keratinocyte serum free medium supplied with epidermal growth factor (EGF). After two days, the medium was changed to keratinocyte basal medium (without EGF) and subsequently cultured for 14 days by the air-liquid interface culture method. We found that time modulation of EGF has great effect on keratinocyte cell behavior. It is suggested that epidermal keratinocytes with bimedium culture system developed the basement membrane and also differentiated upward in the form of multi-layers.
机译：巨大烧伤患者的存活率越来越大，其可用的自动化部位是非常有限的，导致新出现的开发和人工皮肤的要求[1-​​4]。本研究的目的是生产培养的自体角蛋白细胞移植物，并开发出最佳的皮肤替代物的永久性伤口闭合。在皮肤等同物的发展中，将人的皮肤成纤维细胞接种以形成三维皮肤置换组织。最初在提供表皮生长因子（EGF）的角蛋白酶细胞血清可自由培养基中培养角质形成细胞。两天后，将培养基改变为角蛋白细胞基础培养基（无EGF），随后通过气液界面培养方法培养14天。我们发现EGF的时间调制对角蛋白细胞的特性产生了很大的影响。建议具有BimeDium培养系统的表皮角质形成细胞开发了基底膜，并以多层的形式向上区分。

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