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首页> 外文会议>International Plant Tissue Culture Conference >Variations in Betalain Contents among Different Cell Lines and Some Factors Affecting the Biosynthesis of Betalain in Cell Culture of Portulaca sp. cv. 'Jewel'
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Variations in Betalain Contents among Different Cell Lines and Some Factors Affecting the Biosynthesis of Betalain in Cell Culture of Portulaca sp. cv. 'Jewel'

机译：不同细胞系中β基因内容物的变化以及影响Portulaca SP细胞培养物中βain生物合成的一些因素。简历。 '珠宝'

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We have established 16 different colored cell lines of Portulaca sp. cv. 'Jewel' (JR inbred) including 7 lines on modified Murashige and Skoog (mMS) and 9 lines on modified Girod and Zryd (mJl) culture medium. Betalain composition of those cell lineswas determined by using the high performance liquid chromatography -UV-VIS-photodiode array detector (HPLC-UV-VIS-PDA) method. The cell lines were distinctly different on their betalain contents. Betacyanin and betaxanthin, greatly varied in quality andquantity from line to line. N12 mMS-cell line, a magenta color phenotype, contains highest amount (4 87 mg/g FW) of total betacyanin and N12Or mJ1-cell line, an orange color phenotype, contains highest amount (3819 89 mu g/g FW) of total betaxanthin among all the lines. The cells cultured on mMS medium synthesize more betacyanin in comparison with mJ1 medium. In contrast, mJ1 medium cultured cells synthesize more betaxanthin than that of mMS medium. Addition of natural auxin, indole-3-acetic acid (IAA), to both types of culture media instead of synthetic auxin, 2,4-dichloro-phenoxyacetic acid (2,4-D) affected the growth and betalain biosynthesis 6 mu M and 9 mu M IAA significantly increased the betalain content during the first subculture than that ofcontrol in 12 mMS-cell line and 4YmJ1-cell line (yellow color phenotype), respectively. Effects of different light conditions on betalain biosynthesis were also studied with a magenta color and a yellow color cell lines. Continuous blue light treated cells optimistically influenced the betacyanin and betaxanthin contents in both colored cell lines than the cells cultured on continuous white or 16 h/day white light conditions and the rate of cellular betalain contents increased drastically under continuous white and blue light conditions along with the increasing number of growth cycle.
机译：我们已经建立了16种不同的Portulaca SP细胞系。简历。 '珠宝'（JR近交）包括改进的Murashige和Skoog（MMS）的7条线路和改进的Girod和Zryd（MJL）培养基上的9行。通过使用高效液相色谱-MV-Vis光电二极管阵列检测器（HPLC-UV-VIS-PDA）方法，确定这些细胞系的嵌合组成。细胞系在其β含量明显不同。 Betacyanin和Betaxanthin，从线到线的质量和质量大大变化。 N12彩铃 - 细胞系，洋红色表型，含有总Betacyanin和N120or MJ1-Cell系，橙色颜色表型的最高量（4 87mg / g fw），含有最高量（3819 89 mu g / g fw）所有线条之间的总叶毛。与MJ1培养基相比，在MMS培养基上培养的细胞合成更多的甜菜碱。相反，MJ1培养基培养细胞合成比MMS培养基更多的βanthin。添加天然养液，吲哚-3-乙酸（IAA），两种类型的培养基代替合成毒素，2,4-二氯 - 苯氧基乙酸（2,4-D）影响了生长和贝纳莱因生物合成6 mu m和9μMIAA在第一次转移过程中显着增加了β1含量，而不是12毫米 - 细胞系和4MMJ1-细胞系（黄色表型）中的控制。还研究了不同光条件对β-生物合成的影响，用品红色颜色和黄色细胞系进行了研究。连续蓝光处理细胞在两种彩色细胞系中乐观地影响了贝辛胺和βanthin含量，而不是在连续白细胞上培养的细胞或16小时白光条件，并且在连续的白色和蓝色光线条件下大幅增加了细胞贝纳此含量的速率。越来越多的生长周期。

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