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首页> 外文会议>Asian Society for Mitochondrial Research and Medicine >Effects of Gonadotrophin-Releasing Hormone Agonists on Apoptosis of Granulosa Cells
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Effects of Gonadotrophin-Releasing Hormone Agonists on Apoptosis of Granulosa Cells

机译：促性腺症释放激素激动剂对粒细胞凋亡的影响

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Granulosa cells are known to contribute to maturation of oocytes, and most of the growth factors exert their action via granulosa cells. It has been established that granulosa cell death during follicular atresia and luteolysis results from apoptosis. However, the precise mechanistic pathways of granulosa cell apoptosis have not yet been defined. In this study, we determined the proportions of apoptosis in granulosa cells treated with two kinds of gonadotrophin-releasing hormone agonists (GnRHa): buserelin and leuprorelin depot. The incidences of DNA fragmentation of human granulosa cells treated with buserelin and leuprorelin were 54.33% and 39.02%, respectively. The proportions of apoptotic bodies were 6.04% and 4.29%, respectively. There was a significant difference in the proportions of DNA fragmentation between the two kinds of GnRHa-treated granulosa cells. The apoptosis pathway and associated protein expression in granulosa cells treated with GnRHa were also determined. The Bax molecule, a pro-apoptosis protein, was expressed in granulosa cells undergoing apoptosis. In contrast, Bcl-2, an anti-apoptosis protein, could not be detected in the same group of granulosa cells. The distribution of cytochrome c determined via immunostaining showed a diffuse pattern, which most likely indicated that cytochrome c was translocated from mitochondria into the cytoplasm. Western blotting showed the expressions of caspase-9 and caspase-3 in patients' granulosa cells. The GnRHa effects on granulosa cells indicated a higher incidence of DNA fragmentation and apoptotic bodies in the buserelin-treated than in the leuprorelin depot-treated group. The granulosa cells go through the mitochondria-dependent apoptosis pathway; the indicated pro-apoptosis protein Bax was expressed and induced cytochrome c release from mitochondria, which then activated caspase-9 and caspase-3 until cell death occurred.
机译：已知颗粒细胞有助于卵母细胞的成熟，并且大多数生长因子通过颗粒细胞施加其作用。已经建立了卵泡休息期间颗粒细胞死亡和胰溶解来自凋亡的结果。然而，尚未确定颗粒细胞凋亡的精确机械途径。在这项研究中，我们确定了用两种促进的促性腺素释放激素激动剂（GNRHA）治疗的颗粒细胞细胞凋亡的比例：Buserelin和Leuprorelin Depot。用Buserelin和Leuprorelin处理的人颗粒细胞DNA碎片的发生率分别为54.33％和39.02％。凋亡体的比例分别为6.04％和4.29％。两种GNRHA处理的颗粒细胞之间的DNA片段化比例存在显着差异。还测定了用GNRHA处理的颗粒细胞中凋亡途径和相关蛋白表达。 Bax分子是一种促细胞凋亡蛋白在经历细胞凋亡的颗粒细胞中表达。相反，Bcl-2，抗凋亡蛋白不能在同一组颗粒细胞中检测。通过免疫染色测定的细胞色素C的分布显示弥漫性图案，最有可能表明将细胞色素C从线粒体转移到细胞质中。 Western Blotting显示Caspase-9和Caspase-3在患者颗粒细胞中的表达。 GNRHA对甘蓝细胞的影响表明，在牛肝菌蛋白处理的基团中，在Buserelin处理中的DNA碎片和凋亡体的发病率较高。颗粒细胞通过线粒体依赖性凋亡途径;表达了所指示的促细胞凋亡蛋白Bax，并诱导从线粒体释放的细胞色素C释放，然后激活Caspase-9和Caspase-3直至发生细胞死亡。

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