TGF-beta1 siRNA Decreases Expression of TGF-beta1, FGF-2 and Their Receptors in Mouse Dura Cell Culture

机译：TGF-Beta1 siRNA降低了小鼠Dura细胞培养中TGF-β1，FGF-2及其受体的表达

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Increased levels of TGF-beta1 have been identified in prematurely closing cranial sutures in both a murine model and in humans. Therefore, regulating the expression levels of key growth factors or their receptors may provide a non-surgical means of intervention to delay suture fusion. In this study we examine the ability of TGF-beta siRNA to regulate the gene expression of the factors TGF-betal and FGF-2 as well as their receptors TGF-R1, TGF-R2, and FGF-R1 in a mouse dura cell culture. 16nM TGF-beta1 siRNA is capable of reducing TGF-beta1 mRNA expression significantly when harvested 10 days post-transfection, but expression returned to levels found in mock transfected cells by day 14. Similarly, TGF-R2, FGF-2 and FGF-R1 levels showed an initial reduction in expression on day 4. However, all were near control level by day 7.

机译：在鼠模型和人类中，已经在过早关闭颅缝中鉴定了TGF-Beta1水平。因此，调节关键生长因子或其受体的表达水平可提供延迟缝合融合的非手术手段。在这项研究中，我们研究了TGF-βsiRNA调节因子TGF-BETAL和FGF-2的基因表达以及小鼠Dura细胞培养物中的受体TGF-R1，TGF-R2和FGF-R1的能力。 16NM TGF-Beta1 siRNA能够在转染后10天收获10天时显着降低TGF-β1mRNA表达，但表达返回到模拟转染细胞中的水平在14天。类似地，TGF-R2，FGF-2和FGF-R1水平在第4天表达表达初步减少。但是，所有在第7天都接近控制水平。

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《International Society of Craniofacial Surgery》|2007年||共6页
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D.O. BECK; A. FATHI; N. HALLIGAN; A. GOSAIN;
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1. TGF-beta1 RNA interference in mouse primary dura cell culture: downstream effects on TGF receptors, FGF-2, and FGF-R1 mRNA levels. [J] . Gosain AK, Machol JA 4th, Gliniak C, Plastic and reconstructive surgery . 2009,第5期

机译：小鼠原代硬脑膜细胞培养物中的TGF-beta1 RNA干扰：对TGF受体，FGF-2和FGF-R1 mRNA水平的下游影响。
2. Discussion. TGF-beta1 RNA interference in mouse primary dura cell culture: downstream effects on TGF receptors, FGF-2, and FGF-R1 mRNA levels. [J] . Ko SH, Behr B, Longaker MT Plastic and reconstructive surgery . 2009,第5期

机译：讨论。小鼠原代硬脑膜细胞培养物中的TGF-beta1 RNA干扰：对TGF受体，FGF-2和FGF-R1 mRNA水平的下游影响。
3. TGF-beta1, FGF-2, and receptor mRNA expression in suture mesenchyme and dura versus underlying brain in fusing and nonfusing mouse cranial sutures. [J] . Gosain AK, Recinos RF, Agresti M, Plastic and reconstructive surgery . 2004,第6期

机译：在融合和不融合的小鼠颅骨缝线中，缝线间充质和硬脑膜中的TGF-beta1，FGF-2和受体mRNA表达与基础脑相比。
4. TGF-beta1 siRNA Decreases Expression of TGF-beta1, FGF-2 and Their Receptors in Mouse Dura Cell Culture [C] . D.O. BECK, A. FATHI, N. HALLIGAN, International Society of Craniofacial Surgery . 2007

机译：TGF-Beta1 siRNA降低了小鼠Dura细胞培养中TGF-β1，FGF-2及其受体的表达
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TGF-beta1 siRNA Decreases Expression of TGF-beta1, FGF-2 and Their Receptors in Mouse Dura Cell Culture

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