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首页> 外文会议>International Colloquium on Paratuberculosis >Molecular typing of Mycobacterium avium subsp. paratuberculosis strains from different hosts and regions
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Molecular typing of Mycobacterium avium subsp. paratuberculosis strains from different hosts and regions

机译：分子型分子分枝杆菌亚空间。来自不同主持人和地区的副植物菌菌株

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Genotypic differences have been used to characterise isolates of Mycobacterium avium subsp. paratuberculosis (Map), the agent of paratuberculosis in ruminants. The IS1311 Polymerase Chain Reaction-Restriction Endonuclease Analysis (PCR-REA) is a genetic typing technique that distinguishes between C (cattle), S (sheep) and B (bison) type strains of paratuberculosis. This method was used to detect genetic differences between 381 Map isolates from cattle, sheep, goat and bison, coming from distinct regions of Spain, India and United States. In Spain, all 346 bovine isolates, 4 of 10 caprine isolates (40%) and 1 of 12 ovine isolates (8.3%) were of the C type, whereas the other 11 ovine isolates (91.7%) and 6 caprine isolates (60%) were of the S type. All 5 ovine isolates and 6 caprine isolates from India, as well as all 3 isolates from bison {Bison bison) from the USA, were of the B type. This is the first report on the incidence of B type strains in species other than bison, although multiplex Pulsed Field Gel Electrophoresis (PFGE) and Restricted Fragment Length Polymorphism analysis results indicate that they are not the same strain. These results suggest that there are genetic differences between Map isolates related to geographic and host factors that can have a potential for the epidemiological tracing of new paratuberculosis isolates.
机译：基因型差异已被用于表征分枝杆菌亚空间的分离株。解法物，解反演者的伞菌药物。 IS1311聚合酶链反应限制性内切核酸酶分析（PCR-REA）是一种遗传分类技术，可区分C（牛），S（绵羊）和B（北美野牛）型拮抗菌株。该方法用于检测来自西班牙，印度和美国的不同地区的牛，绵羊，山羊和野牛的381张地图分离物之间的遗传差异。在西班牙，所有346个牛分离物，10个甲状腺分离物（40％）和12个羊株分离物（8.3％）的4个，而另外11个绵羊分离物（91.7％）和6个葡萄酒分离株（60％）（60％ ）是s型。所有5个绵羊分离物和来自印度的6个Caprine分离物，以及来自美国的北美野牛（野牛野牛）的所有3个分离物都是B型。这是关于Bis米以外的物种的B型菌株发病率的第一个报告，但多重脉冲场凝胶电泳（PFGE）和受限制的片段长度多态性分析结果表明它们不是相同的菌株。这些结果表明，与地理和宿主因子相关的地图分离株之间存在遗传差异，这可能对新的副植物分离株的流行病学追踪有潜力。

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