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首页> 外文会议>International Colloquium on Paratuberculosis >Fecal shedding of Mycobactehum avium subsp. paratuberculosis in calves: implications for infection control and management
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Fecal shedding of Mycobactehum avium subsp. paratuberculosis in calves: implications for infection control and management

机译：粪便疫苗亚蚊子患者的粪便脱落。犊牛的Paratuberculosis：对感染控制和管理的影响

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It is widely accepted that most infections caused by Mycobactehum avium subsp. paratuberculosis (MAP) occur in utero or in the neonatal animal. The challenge, however, has been to detect the infection in the young animal to facilitate more prudent animal management decisions. The objectives of this study are to determine whether: 1) fecal shedding of MAP can be detected in naturally infected calves, 2) there is a relationship between MAP test positive cows (ELISA or fecal) and fecal shedding in their offspring, 3) there is an association between fecal shedding in the calf and their ELISA test status, and 4) pooled fecal sample testing is sensitive enough to detect MAP shedding in calves. This is a two year longitudinal, prospective study using dairy calves of four age groups from seven herds in Michigan with known MAP prevalence. Fecal, and blood samples, are obtained from ten calves in each of the four age groups at 3 month intervals. The TREK~R liquid culture system is used for fecal culture and the Biocor~R ELISA for blood. Preliminary results (study 40% complete) showed fecal shedding was detectable in calves with a higher proportion of shedding calves being from ELISA or culture-positive dams. Few calves were ELISA positive (2.2%). There was an association between ELISA positive calves and dam test status but little association with concomitant calf fecal status. Also, pool size may be critical in detection of fecal shedding in calves, with five calves per pool being more sensitive than ten calves per pool.
机译：众所周知，大多数感染患者患有肌卵虫牛亚普。副植物（MAP）发生在子宫或新生儿动物中。然而，挑战一直是检测年轻动物的感染，以促进更谨慎的动物管理决策。本研究的目标是确定：1​​）MAP的粪便脱落可以在天然感染的牛犊中检测到，2）地图测试阳性奶牛（ELISA或FECAL）之间存在关系，并在其后代粪便缩小，3）在那里是小牛和他们的ECISA测试状态的粪便脱落之间的关联，4）汇集的粪便样本测试足以检测小腿的地图脱落。这是一项两年的纵向，前瞻性研究，使用来自密歇根州的七个患者的四岁群体的乳制品犊牛，具有已知地图普遍存在。粪便和血液样本，以3个月间隔在四个年龄组中的每一个中获得10个小牛。 Trek〜R液体培养系统用于粪便培养和生物血管〜R ELISA。初步结果（研究40％完成）显示粪便脱落，犊牛可检测到较高比例的脱落小牛，来自ELISA或培养阳性水坝。少量犊牛是ELISA阳性（2.2％）。 ELISA正面小牛和大坝测试状态之间存在关联，但与伴随小牛粪便的关系很小。此外，池大小对于检测犊牛的粪便脱落可能是至关重要的，每个池5个小腿比每池更敏感。

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