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High sequence coverage by in-capillary proteolytic digest: identification and/or sequencing of hemoglobins directly from blood samples

机译：毛细血管内蛋白水解消化的高序列覆盖：直接从血液样品鉴定和/或血红蛋白排序

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We recently developed a method for direct identification and/or sequencing of proteins by proteolytic digest in the electrospray capillary and simultaneous analysis of the resulting peptides by nanoESI MS and MS/MS [1]. Since this strategy yields high sequence coverages it was now applied to the analysis of hemoglobins (Hbs) directly from diluted blood samples.

机译：我们最近通过电喷雾毛细管中的蛋白水解消解和通过纳米MS和MS / MS进行同时分析所得肽的蛋白水解消化来直接鉴定和/或测序蛋白质的方法。由于该策略产生高序列覆盖，因此它现在应用于直接从稀释的血液样品分析血红蛋白（HBS）。

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2006年||共2页
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G. Pohlentz; S. Henning; M. Mormann; J. Peter-Katalinic;
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中图分类 TB994.5-53;
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1. Direct analysis of α- and β-chains of hemoglobins from mammalian blood samples by nanoESI mass spectrometry during in-capillary proteolytic digestion [J] . Stefanie Henning, Michael Mormann, Jasna Peter-Katalinic Amino acids . 2011,第2期

机译：在毛细管内蛋白水解过程中通过nanoESI质谱法直接分析哺乳动物血样中血红蛋白的α和β链
2. Direct analysis of α- and β-chains of hemoglobins from mammalian blood samples by nanoESI mass spectrometry during in-capillary proteolytic digestion [J] . Stefanie Henning, Michael Mormann, Jasna Peter-Katalinić, Amino Acids . 2011,第2期

机译：在毛细管内蛋白水解过程中通过nanoESI质谱法直接分析哺乳动物血样中血红蛋白的α和β链
3. Broad coverage identification of multiple proteolytic cleavage site sequences in complex high molecular weight proteins using quantitative proteomics as a complement to edman sequencing. [J] . Doucet A, Overall CM Molecular & cellular proteomics: MCP . 2011,第5期

机译：使用定量蛋白质组学作为edman测序的补充，对复杂的高分子量蛋白中的多个蛋白水解切割位点序列进行广泛的鉴定。
4. High sequence coverage by in-capillary proteolytic digest: identification and/or sequencing of hemoglobins directly from blood samples [C] . G. Pohlentz, S. Henning, M. Mormann, ASMS Conference on Mass Spectrometry and Allied Topics . 2006

机译：毛细血管内蛋白水解消化的高序列覆盖：直接从血液样品鉴定和/或血红蛋白排序
5. A selective whole genome amplification and sequencing directly from an environmental sample of Coxiella burnetii. [D] . Cocking, Jill Hager. 2016

机译：直接从伯氏柯氏杆菌的环境样品中进行选择性的全基因组扩增和测序。
6. Broad Coverage Identification of Multiple Proteolytic Cleavage Site Sequences in Complex High Molecular Weight Proteins Using Quantitative Proteomics as a Complement to Edman Sequencing [O] . Alain Doucet, Christopher M. Overall 2011

机译：使用定量蛋白质组学作为埃德曼测序的补充对复杂的高分子量蛋白质中的多个蛋白水解切割位点序列进行广泛的鉴定
7. Broad Coverage Identification of Multiple Proteolytic Cleavage Site Sequences in Complex High Molecular Weight Proteins Using Quantitative Proteomics as a Complement to Edman Sequencing* [O] . Doucet, Alain, Overall, Christopher M. 2010

机译：使用定量蛋白质组学作为埃德曼测序的补充，对复杂的高分子量蛋白质中的多个蛋白水解切割位点序列进行广泛的鉴定*

High sequence coverage by in-capillary proteolytic digest: identification and/or sequencing of hemoglobins directly from blood samples

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