Performance of Polystyrene-Divinylbenzene Monolithic ESI Needles for Nano LC-MS/MS Peptide Sequencing

机译：聚苯乙烯 - 二乙烯基苯单片针针对纳米LC-MS / MS肽测序的性能

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Peptide sequencing by Nano LC-ESI-MS/MS is the main technique for protein identification in proteomics. Typically a protein digest sample is separated in one hour, employing 75 (mu)m ID packed nano columns. For large-scale proteomics analysis this approach is rather time consuming and therefore typically applied for samples that do not reveal enough information with MALDI-MS. With the chromatographic separation as being the time limiting factor Polystyrene-divinylbenzene (PS-DVB) monolithic supports can reduce the LC-MS/MS analysis time significantly. Integration of the PS-DVB monolithic column and electrospray needle provides a dead-volume free and highly efficient separation system for peptides and proteins.

机译：纳米LC-ESI-MS / MS的肽测序是蛋白质组学中蛋白质鉴定的主要技术。通常，在一小时内分离蛋白质消化样品，采用75（mU）M ID填充纳米柱。对于大规模蛋白质组学分析，这种方法在耗时的耗时，因此通常适用于没有用MALDI-MS透露足够信息的样本。随着色谱分离作为时间限制因子聚苯乙烯 - 二乙烯基苯（PS-DVB）整体载体可以显着降低LC-MS / MS分析时间。 PS-DVB单片柱和电喷雾针的集成为肽和蛋白质提供了无型无效和高效的分离系统。

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2006年||共2页
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Mark van Gils; Robert van Ling; Bjorn de Haan; Bas Dolman; Remco Swart;
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中图分类 TB994.5-53;
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Performance of Polystyrene-Divinylbenzene Monolithic ESI Needles for Nano LC-MS/MS Peptide Sequencing

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