首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Identification of Acylation Patterns and Novel Modifications in Lipid A species from Francisella using a Vacuum-MALDI LTQ Linear Ion Trap

【24h】

Identification of Acylation Patterns and Novel Modifications in Lipid A species from Francisella using a Vacuum-MALDI LTQ Linear Ion Trap

机译：使用真空-Maldi LTQ线性离子阱鉴定Francisella的脂质脂质的酰化模式和新改性

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

Francisella tularensis is a gram-negative, non-sporulating, encapsulated coccobacillus that causes tularemia. F. tularensis is transmitted to humans by the bite of infected insects or animals, ingestion of contaminated water or food, and by inhalation of aerosolized particles. This latter method of infection can cause disease with high morbidity and mortality. In this study we are investigating a novel F. tularensis lipid A late acyltransferase (LpxL) performing complementation studies in E. coli and generating a LpxL mutant in F. tularensis. Further studies are presented of lipid A moieties from lipopolysaccharide (LPS) obtained from F. tularensis, F. novicida, and F. philomiragia. It has been shown that the degree of acylation and other modifications of lipid A influence toxicity. We have developed sensitive mass spectrometric techniques to identify novel low abundance, high mass lipid A structures, unravel their complex acylation states, and characterize unusual sugar modifications.

机译：Francisella Tularensis是一种革兰氏阴性的非孢子，包封的封装的Coccobacillus，导致Tularemia。 F. Tularensis通过感染的昆虫或动物的叮咬传播给人类，摄入污染的水或食物，并通过吸入雾化颗粒。后一种感染方法会导致发病率高和死亡率高的疾病。在该研究中，我们正在研究一种新的F.Tularensis脂质在大肠杆菌中进行辅酰转移酶（LPX1）并在F.Tularensis中产生LPXL突变体。从F.Tultensis，F. Novicida和F. Philomiragia获得的脂多糖（LPS）提出了进一步的研究。已经表明，酰化程度和脂质的其他修饰影响毒性。我们已经开发了敏感的质谱技术，以识别新型低丰度，高质量脂质的结构，解开它们的复杂酰化状态，并表征异常的糖修改。

著录项

来源
《ASMS Conference on Mass Spectrometry and Allied Topics》|2006年||共2页
会议地点
作者
Birgit Schilling; Molly K. McLendon; Nancy J. Phillips; Michael A. Apicella; Bradford W. Gibson;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种
中图分类 TB994.5-53;
关键词

相似文献

外文文献
中文文献
专利

1. Comparison of triple quadrupole, hybrid linear ion trap triple quadrupole, time-of-flight and LTQ-Orbitrap mass spectrometers in drug discovery phase metabolite screening and identification in vitro — amitriptyline and verapamil as model compounds [J] . Timo Rousu, Jukka Herttuainen, Ari Tolonen Rapid Communications in Mass Spectrometry: RCM . 2010,第7期

机译：三重四极杆，混合线性离子阱三重四极杆，飞行时间和LTQ-Orbitrap质谱仪在体外药物发现阶段代谢物筛选和鉴定中的比较-阿米替林和维拉帕米为模型化合物
2. Identification and fragmentation pathways of caffeine metabolites in urine samples via liquid chromatography with positive electrospray ionization coupled to a hybrid quadrupole linear ion trap (LTQ) and Fourier transform ion cyclotron resonance mass spectrometry and tandem mass spectrometry [J] . Bianco G, Abate S, Labella C, Rapid Communications in Mass Spectrometry: RCM . 2009,第7期

机译：尿液中咖啡因代谢物的鉴定和裂解途径：液相色谱-正电喷雾电离耦合四极杆线性离子阱（LTQ）和傅里叶变换离子回旋共振质谱和串联质谱
3. Identification of glucosinolates in capers by LC-ESI-hybrid linear ion trap with Fourier transform ion cyclotron resonance mass spectrometry (LC-ESI-LTQ-FTICR MS) and infrared multiphoton dissociation [J] . Bianco G., Lelario F., Battista F.G., Journal of mass spectrometry: JMS . 2012,第9期

机译：LC-ESI-混合线性离子阱，傅立叶变换离子回旋共振质谱（LC-ESI-LTQ-FTICR MS）和红外多光子离解法鉴定刺山柑中的芥子油苷
4. Identification of Acylation Patterns and Novel Modifications in Lipid A species from Francisella using a Vacuum-MALDI LTQ Linear Ion Trap [C] . Birgit Schilling, Molly K. McLendon, Nancy J. Phillips, ASMS Conference on Mass Spectrometry and Allied Topics . 2006

机译：使用真空-Maldi LTQ线性离子阱鉴定Francisella的脂质脂质的酰化模式和新改性
5. Contrasting patterns of transposable element insertion polymorphism in autotetraploid and allotetraploid Arabidopsis species [D] . Hazzouri, Khaled 2007

机译：同源四倍体和异源四倍体拟南芥种中转座子插入多态性的对比模式
6. Characterization of Lipid A Acylation Patterns in Francisella tularensis F. novicida and F. philomiragia using Multiple-Stage Mass Spectrometry (MSn) on a vMALDI Linear Ion Trap [O] . Birgit Schilling, Molly K. McLendon, Nancy J. Phillips, -1

机译：在vMALDI线性离子阱上使用多阶段质谱（MSn）表征土拉弗朗西斯菌新孢子虫和费氏衣藻的脂质A酰化模式
7. Characterization of Lipid A Acylation Patterns in Francisella tularensis, Francisella novicida, and Francisella philomiragia Using Multiple-Stage Mass Spectrometry on a Matrix-Assisted Laser Desorption/Ionization on a Vacuum Source Linear Ion Trap [O] . -1

机译：在真空源线阱上的矩阵辅助激光解吸/电离上使用多阶段质谱，Francisella Tularityisis，Francisella Novicida，Francisella和Francisella Philomiragia的脂质酰化模式的表征

Identification of Acylation Patterns and Novel Modifications in Lipid A species from Francisella using a Vacuum-MALDI LTQ Linear Ion Trap

摘要

著录项

相似文献

相关主题

期刊订阅