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首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Sample Preparation and Protein Removal: A Comparison of Protein Removal with Various Sample Preparation Techniques using Gel Electrophoresis
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Sample Preparation and Protein Removal: A Comparison of Protein Removal with Various Sample Preparation Techniques using Gel Electrophoresis

机译：样品制备和蛋白质去除：使用凝胶电泳的各种样品制备技术的蛋白质去除比较

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摘要

Protein precipitation shows far greater protein levels for crash ratios below the suggested 1:3 (v/v) ratio. Increasing the crash ratio to 1:6 did not improve protein removal. A rough protein quantitation (n(velence)1) showed greater than 99percent of total protein removed using PPT with the ISOLUTE PPT+ generic method. Supported Liquid Extraction, as expected, had no protein in the final extract. This is due to the low solubility of proteins in the water immiscible extraction solvent, MTBE. Silica-based non-polar SPE C8 gave the lowest protein levels out of the various chain lengths. This could be due to a better balance between the hydrophobic and steric effects of the C8 compared to the others. The MFC18 shows considerably more protein retention than the standard C18 product, indicating that pore size has a substantial effect on protein retention.
机译：蛋白质沉淀显示出低于建议的1：3（v / v）比率的碰撞比的更大蛋白质水平。将崩溃比率提高到1：6没有改善蛋白质去除。粗蛋白质定量（N（柔软）1）显示使用PPT与ISOLUTE PPT +通用方法除去的总蛋白质的99％。如所预期的，支持的液体提取在最终提取物中没有蛋白质。这是由于蛋白质在水不混溶的萃取溶剂中的溶解度低，MTBE。基于二氧化硅的非极性SPE C8将最低的蛋白质水平放出各种链长。这可能是由于与其他相比，C8的疏水性和空间效应之间的更好平衡。 MFC18显示出比标准C18产物更多的蛋白质保留，表明孔径对蛋白质保留具有显着影响。

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