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De Novo Sequencing of E.coli Lysate Using a Single Series of Fragment Ions via Chromophore Tagging and 351 nm UVPD

机译:通过发色团标签使用单一系列片段离子和351 nm UVPD使用单一系列片段离子的Novo测序

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Selective derivatization of the N-termini of tryptic peptides with a UV chromophore (AMCA) results in UVPD mass spectra that contain predominantly y ions. UVnovo combines a random forest classifier and a Hidden Markov model to simplify and interpret UVPD fragmentation spectra enabling the de novo sequencing of thousands of peptides from an E. coli lysate at high confidence. UVnovo identified 47.7% of the sequences correctly when only the top result was considered and 58.2% when the top three results are considered.
机译:用UV发色团(AMCA)的胰蛋白酶肽的N-末端的选择性衍生化导致UVPD质谱,其主要含有Y离子。 UVNOVO结合了随机森林分类器和隐藏的马尔可夫模型,简化和解释了UVPD碎片光谱,使得在高信心高度裂解物中可以从大肠杆菌裂解物中进行数千种肽。 UVNOVO在考虑最高结果时正确鉴定了47.7%的序列,并且当考虑前三个结果时58.2%。

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