LC-MS/MS for the Quantitative Measurement of 8,5'-Cyclopurine-2'-Deoxynucleosides in DNA

机译：LC-MS / MS用于定量测量8,5'-环尿-2'-脱氧核苷的DNA

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8,5′-cyclopurine-2′-deoxynucleosides (cPus) are formed in DNA upon exposure to hydroxyl radicals. cPus can block DNA replication and transcription, causing replication and transcriptional mutagenesis, and are considered reliable biomarkers of oxidative stress and oxidatively induced DNA damage. With the increasing demand for sensitive and rapid quantification of cPus in vivo, we are in the process of developing a nanoflow LC-MS/MS-based method for the quantitative measurement of cPus using a few micrograms of DNA samples that does not require the labor-intensive off-line HPLC enrichment procedures.

机译：在暴露于羟基自由基时，在DNA中形成8,5'-环嘌呤-2'-脱氧核苷（CPU）。 CPU可以阻断DNA复制和转录，导致复制和转录诱变，并且被认为是可靠的氧化应激和氧化诱导的DNA损伤的生物标志物。随着对体内CPU的敏感和快速量化的需求不断增加，我们正在开发基于纳米射线LC-MS / MS的方法，用于使用一些不需要劳动的DNA样品进行定量测量CPU的定量测量 - 抗性离线HPLC浓缩程序。

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2016年|1CD|共1页
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Yuxiang Cui; Laura J. Niedernhofer; Yinsheng Wang;
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中图分类 TB994.5-53;
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1. 8,5'-Cyclopurine-2'-deoxynucleosides in DNA: Mechanisms of formation, measurement, repair and biological effects. [J] . Jaruga P, Dizdaroglu M DNA repair . 2008,第9期

机译：DNA中的8,5'-环嘌呤2'-脱氧核苷：形成，测量，修复和生物学效应的机制。
2. Mutagenicity and genotoxicity of (5′ s)-8,5′-cyclo-2′- deoxyadenosine in escherichia coli and replication of (5′ s)-8,5′-cyclopurine-2′-deoxynucleosides in vitro by dna polymerase iv, exo-free klenow fragment, and dpo4 [J] . PednekarV., WeerasooriyaS., JastiV.P., Chemical research in toxicology . 2014,第2期

机译：（5's）-8,5'-cyclo-2'-脱氧腺苷在大肠杆菌中的致突变性和遗传毒性以及（5's）-8,5'-cyclopurine-2'-deoxynucleosides的体外DNA聚合酶复制，无exo的klenow片段和dpo4
3. Translesion Synthesis of 8,5′-Cyclopurine-2′-deoxynucleosides by DNA Polymerases η, ι, and ζ [J] . Changjun You, Ashley L. Swanson, Xiaoxia Dai, The Journal of biological chemistry . 2013,第40期

机译：通过DNA聚合酶η，1和ζ的8,5'-环嘌呤-2'-脱氧核苷的翻塑合成
4. LC-MS/MS for the Quantitative Measurement of 8,5'-Cyclopurine-2'-Deoxynucleosides in DNA [C] . Yuxiang Cui, Laura J. Niedernhofer, Yinsheng Wang ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：LC-MS / MS用于定量测量8,5'-环尿-2'-脱氧核苷的DNA
5. LC-MS/MS/MS Quantification of Oxidatively Induced DNA Lesions [D] . Guerrero, Candace Rae 2014

机译：LC-MS / MS / MS定量测定氧化性DNA损伤
6. Development and validation of a LC-MS/MS quantitative method for endogenous deoxynucleoside triphosphates in cellular lysate [O] . Xinhui Chen, Kevin J. McAllister, Brandon Klein, -1

机译：裂解液中内源性脱氧核苷三磷酸的LC-MS / MS定量方法的开发和验证
7. Accurate and Efficient Bypass of 8,5′-Cyclopurine-2′-Deoxynucleosides by Human and Yeast DNA Polymerase η [O] . Ashley L. Swanson, Jianshuang Wang, Yinsheng Wang 2012

机译：通过人和酵母DNA聚合酶η准确高效地旁路8,5'-环尿-2'-脱氧核苷

LC-MS/MS for the Quantitative Measurement of 8,5'-Cyclopurine-2'-Deoxynucleosides in DNA

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