首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Simultaneous Measurement of 25-Hydroxyvitamin-D2, 25-Hydroxyvitamin-D3 and 27-Hydroxycholesterol Levels in Human Serum by LC-MS/MS
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Simultaneous Measurement of 25-Hydroxyvitamin-D2, 25-Hydroxyvitamin-D3 and 27-Hydroxycholesterol Levels in Human Serum by LC-MS/MS

机译:通过LC-MS / MS在人血清中同时测量25-羟基vitamin-d2,25-羟基vitamin-d3和27-羟基胆固醇水平

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A sensitive and robust liquid chromatography–tandem quadrupole mass spectrometry (LC–MS/MS) method was developed for simultaneous measurement of 25HVD2, 25HVD3, and total 27HC in human serum. A core shell C18 LC column with sub-2 μm particles (PhenomenexKinetex C18) was used to enhance chromatographic separation and achieve higher resolution and peak capacity.
机译:开发了一种敏感和鲁棒的液相色谱 - 串联四极谱(LC-MS / MS)方法,用于同时测量25HVD2,25HVD3和18HC的人血清中的27HC。使用具有Sub-2μm颗粒的核心壳C18LC柱(现象Xkinetex C18)来增强色谱分离并实现更高的分辨率和峰值容量。

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