• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Difference Mass Spectra for De novo Identification of N- and C-terminal Sequences of Intact Proteins
【24h】

Difference Mass Spectra for De novo Identification of N- and C-terminal Sequences of Intact Proteins

机译：完整蛋白N-和C末端序列DE Novo鉴定的差异质谱

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The gene sequence encoding the variable regions of antibodies are produced via V(D)J recombination followed by somatic hypermutation. The vast number combinatorial sequences generated for unknown clones make antibody amino acid sequence determination rather complex [1]. Next generation gene sequencing capabilities has enabled proteogenomic approaches in assisting the identification of variable regions of antibody repertoires [2]. However, the gold standard for sequencing of complement determining regions (CDR) of antibodies are by Edman degradation or de novo sequencing by alignment of peptide fragment generated using multiple proteases [3]. Here we introduce a difference spectral method that will assist in the de novo identification of both the N-terminus and C-terminus ends of intact proteins subjected to top-down mass spectrometry. All experiments were performed on an Orbitrap Fusion mass spectrometer using commercially available proteins such as Histone H3.1 and SILu monoclonal antibody standard obtained from Sigma.
机译：编码抗体可变区的基因序列通过V（d）J重组产生，然后进行体细胞高级抵抗。为未知克隆产生的庞大数组序列使抗体氨基酸序列测定相当复杂[1]。下一代基因测序能力使得能够在辅助抗体曲目的可变区的鉴定方面使突畴方法[2]。然而，通过Edman降解或通过使用多种蛋白酶产生的肽片段对准[3]，通过Edman降解或De Novo测序来测序抗体的补体确定区域（CDR）的金标准。在这里，我们介绍一种差异光谱法，该方法将有助于N-Terminus和C-末端的DE Novo鉴定完整蛋白质的N-末端和C-末端进行对外光谱法的完整蛋白质。使用商业上可获得的蛋白质如组蛋白H3.1和从Sigma获得的Silu单克隆抗体标准，对所有实验进行了所有实验。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号