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首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >LC-MS/MS analysis of Diarrhetic Shellfish Poisoning and Azaspiracid Shellfish Poisoning biotoxins
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LC-MS/MS analysis of Diarrhetic Shellfish Poisoning and Azaspiracid Shellfish Poisoning biotoxins

机译：LC-MS / MS分析腹泻贝类中毒和Azaspiracid贝类中毒生物毒素

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Marine biotoxins, produced by microalgae, are small molecules accumulated in filter-feeding fish or shellfish. Consumption of seafood contaminated with marine biotoxins by humans can lead to symptoms such as diarrhea, vomiting, paralysis and even death. These biotoxins therefore impact public health in multiple aspects including regulatory agencies, aquaculture, fisheries and international trade. Diarrhetic shellfish poisoning (DSP) and azaspiracid shellfish poisoning (AZP) are emerging issues in the US following recent DSP outbreaks in US shellfish harvesting areas and an AZP outbreak from imported seafood. To reach the goal of continually monitoring for DSTs and AXTs in the US seafood supply, in this study, a LC-MS/MS method is developed to detect both DSP and AZP marine biotoxins. The method was modified from an official European Union-approved protocol designed for the detection of several classes of EU-regulated lipophilic toxins. A liquid chromatography triple quadrupole mass spectrometry method for the separation and detection of prominent DSP and AZP marine biotoxin groups is described in this abstract. Separation was achieved by a C18 Hypersil gold column (50mm × 2mm, 1.9μm particle size) with a single run of 7.5 minutes. The mobile phase is comprised of a gradient acetonitrile/water containing ammonium formate and formic acid. Both positive and negative modes have been used for multiple reaction monitoring with a Thermo triple quadrupole (TSQ Quantum Ultra) mass spectrometer. The limit of quantification of okadaic acid, dinophysistoxin (DTX)1, DTX2, Azaspiracid (AZA) 1, AZA2 and AZA3 are 9.70, 9.53, 9.70, 2.32, 2.28 and 2.36 fmol, respectively. The method shows sufficient sensitivity, linearity and reproducibility. We conclude that this LC/MS-MS method could provide rapid testing for DSP and AZA biotoxins and is a viable strategy in monitoring selected types of marine toxins in the seafood supply.
机译：由微藻产生的海洋生物毒素是在过滤鱼或贝类中积累的小分子。用人类的海洋生物毒素污染的海鲜消耗可能导致腹泻，呕吐，瘫痪甚至死亡等症状。因此，这些生物毒素在包括监管机构，水产养殖，渔业和国际贸易的多个方面影响公共卫生。腹泻贝类中毒（DSP）和Azaspiracid贝类中毒（AZP）在美国贝类爆发中，美国贝类收获区域的近期爆发和进口海鲜的AZP爆发出现了美国。为了达到在美国海鲜供应中不断监测DSTS和AXTS的目标，在本研究中，开发了LC-MS / MS方法以检测DSP和AZP海洋生物毒素。该方法由官方欧盟批准的官方批准的协议修改，专为检测多种欧盟滋肝菌毒素。液相色谱三重四极杆质谱法用于分离和检测突出的DSP和AZP海洋生物毒素组的分离和检测。通过C18 Hypersil金柱（50mm×2mm，1.9μm粒径）实现分离，单一的7.5分钟。流动相由含有甲酸铵和甲酸的梯度乙腈/水组成。正极和负模式都已用于多重反应监测，具有热Triple四极（TSQ量子超）质谱仪。冈田酸，Dinophysistoxin（DTX）1，DTX2，AzaspiraciD（AZA）1，AZA2和AZA3的定量极限分别为9.70,9.53,9.70,2.32,2.28和2.36 fmol。该方法显示出足够的灵敏度，线性和再现性。我们得出结论，该LC / MS-MS方法可以为DSP和AZA生物毒素提供快速检测，并且是在海产品供应中监测选定类型的海洋毒素的可行策略。

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