Deoxynivalenol (DON) is a most common myco-toxin in grains, which is produced by Fusarium species and is toxic for pigs already at low concentrations (1,2). Biological samples such as serum are complex matrices and thus prone to exert interferences when analyzed by diverse methods. The aim of the project was to establish extraction, cleanup, and analytical methods (HPLC, ELISA) with respect to DON-analysis, which are just partly validated for serum. Additionally, the validity of the ELISA technique was tested in comparison to HPLC, as ELISAs are simple, fast, and require less material and equipment efforts.
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