Fusarium spp. are able to infect several agricultural crops and produce mycotoxins as deoxynivalenol (DON). DON absorption in pigs is rapid and nearly complete. The main site of DON absorption is the small intestine, a considerable smaller part is finally absorbed in the large intestine (1). The tissue distribution showed higher concentrations in plasma, liver and kidney than in other tissues (2). Urine could be defined as the main excretion route for DON. DON is hardly metabolised in pigs, about 95% of the administered dose could be recovered as DON (3). The aim of this study was to develop clean up, extraction and detection methods for DON in urine and to compare HPLC and ELISA results.
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