Engineering a C-terminally modified human flavin containing monooxygenase

机译：工程含有单氧基酶的C末端改性人黄素

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

Human flavin containing monooxygenase 3 is a drug-metabolizing enzyme involved in the oxidation of many drugs, chemicals and xenobiotics. It is predominantly expressed in the liver where its substrates, generally soft nucleophiles are transformed into more polar metabolites. The enzyme is attached to the smooth endoplasmic reti-culum membrane, where its reduced flavin group binds molecular oxygen and readily performs nucleophilic attack on the substrates. To date the structure of hFMO3 has not been solved due to difficulties in the crystallization of microsomal, membrane-bound proteins. In the present study a molecular model of hFMO3 is constructed, exploited by in silico molecular dynamics experiments to identify the membrane anchor and used for the rational design of a soluble truncated hFMO3 enzyme.

机译：含有单氧化酶3的人黄素是涉及许多药物，化学品和异种菌药的氧化的药物代谢酶。它主要在肝脏中表达，其中其基材，通常软的亲核试剂转化为更偏极的代谢物。酶附着在光滑的内质复制膜上，其减少的黄素基团结合分子氧，并且容易对基板进行亲核攻击。迄今为止，由于微粒体，膜结合蛋白质的结晶困难，HFMO3的结构尚未解决。在本研究中，通过在硅分子动力学实验中实施HFMO3的分子模型，以鉴定膜锚并用于可溶性截短的HFMO3酶的合理设计。

著录项

来源
《International Conference on Cytochrome P450》|2011年||共6页
会议地点
作者
Catucci G.; Sadeghi S. J.; Gilardi G.;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种
中图分类氧化还原酶;
关键词

相似文献

外文文献
中文文献
专利

1. In vitro drug metabolism by C-terminally truncated human flavin-containing monooxygenase 3 [J] . CatucciG., GilardiG., JeukenL., Biochemical Pharmacology . 2012,第4期

机译：C端截短的人类含黄素的单加氧酶3的体外药物代谢
2. Metabolism and Disposition of a Novel Selective alpha 7 Neuronal Acetylcholine Receptor Agonist ABT-126 in Humans: Characterization of the Major Roles for Flavin-Containing Monooxygenases and UDP-Glucuronosyl Transferase 1A4 and 2B10 in Catalysis [J] . Liu Hong, Stresser David M., Michmerhuizen Melissa J., Drug Metabolism and Disposition: The Biological Fate of Chemicals . 2018,第4期

机译：一种新的选择性α7神经元乙酰胆碱受体激动剂ABT-126的代谢和配置：表征含有黄素的单氧基酶和UDP-葡糖糖基转移酶1A4和2B10在催化中的主要作用
3. Metabolism and Disposition of a Novel Selective alpha 7 Neuronal Acetylcholine Receptor Agonist ABT-126 in Humans: Characterization of the Major Roles for Flavin-Containing Monooxygenases and UDP-Glucuronosyl Transferase 1A4 and 2B10 in Catalysis (vol 46, pg 429, 2018) [J] . Liu H., Stresser D. M., Michmerhuizen M. J., Drug Metabolism and Disposition: The Biological Fate of Chemicals . 2018,第9期

机译：一种新型选择性α的新生乙酰胆碱受体激动剂ABT-126在人体中的代谢和处理：表征含有黄素的单氧基酶和UDP-葡糖糖基转移酶1A4和2B10在催化中的主要作用（Vol 46，PG 429,2018）
4. Engineering a C-terminally modified human flavin containing monooxygenase [C] . Catucci G., Sadeghi S. J., Gilardi G. International Conference on Cytochrome P450 . 2011

机译：工程含有单氧基酶的C末端改性人黄素
5. Selectivity Mechanisms Employed by Flavin-Dependent Monooxygenases [D] . Benitez, Attabey Rodríguez. 2021

机译：Flavin依赖性单氧基酶采用的选择性机制
6. Structural and kinetic insights into flavin-containing monooxygenase and calponin-homology domains in human MICAL3 [O] . Junsoo Kim, Haemin Lee, Yeon Jin Roh, 2020

机译：对人MICAL3中含有黄素的单加氧酶和钙还原蛋白同源域的结构和动力学的了解
7. Correction to “Metabolism and Disposition of a Novel Selective α7 Neuronal Acetylcholine Receptor Agonist ABT-126 in Humans: Characterization of the Major Roles for Flavin-Containing Monooxygenases and UDP-Glucuronosyl Transferase 1A4 and 2B10 in Catalysis” [O] . 2018

机译：校正“一种新型选择性α7神经元乙酰胆碱受体激动剂ABT-126中的代谢和配置：表征含有黄素的单氧基酶和UDP-葡糖糖基转移酶1A4和2B10在催化中的主要作用”

Engineering a C-terminally modified human flavin containing monooxygenase

摘要

著录项

相似文献

相关主题

期刊订阅