MRM QUANTIFICATION OF HOST CELL PROTEINS IN PROTEIN BIOPHARMACEUTICALS

机译：蛋白质生物制药中宿主细胞蛋白的MRM定量

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

The LC/MS~(E)-based assay allows the identification and quantification of low-abundance HCP contaminants in biopharmaceuticals over four orders of magnitude in concentration. TRIZAIC nanoTile in combination with the TQ-S tandem quadrupole shows great promise for high-throughput HCP quantification or monitoring (under 10 min runtime). Protein A purification of mAbs using different purification protocols produces different HCP patterns.

机译：基于LC / MS〜（e）的测定允许在生物制药中鉴定和定量生物制药中的低丰度HCP污染物超过四个级别的级别。 Trizaic Nanitile与TQ-S Tandem四极杆联合使用，对高通量HCP量化或监测（10分钟运行时）表示伟大的承诺。使用不同纯化方案纯化MAb的纯化产生不同的HCP模式。

著录项

来源
《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2011年||共1页
会议地点
作者
Catalin E. Doneanu; Weibin Chen; Alex Xenopoulos; Jim Murphy; Keith Fadgen; StJohn Skilton; Jeff Mazzeo;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种
中图分类 TB994.5-53;
关键词

相似文献

外文文献
中文文献
专利

1. Targeted Host Cell Protein Quantification by LC-MRM Enables Biologics Processing and Product Characterization [J] . Gao Xinliu, Rawal Baibhav, Wang Yi, Analytical chemistry . 2020,第1期

机译：LC-MRM的靶向宿主细胞蛋白质量化使生物学处理和产品表征能够实现
2. Overcoming biofluid protein complexity during targeted mass spectrometry detection and quantification of protein biomarkers by MRM cubed (MRM~3) [J] . Jeremy Jeudy, Arnaud Salvador, Romain Simon, Analytical and bioanalytical chemistry . 2014,第4期

机译：通过MRM立方（MRM〜3）在目标质谱检测和蛋白质生物标志物定量过程中克服了生物流体蛋白质的复杂性
3. Improved identification of host cell proteins in a protein biopharmaceutical by LC-MS/MS using the ProteoMiner (TM) Enrichment Kit [J] . Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis . 2020,第期

机译：使用蛋白质小甲蛋白酶（TM）富集试剂盒改善蛋白质生物药物中宿主细胞蛋白的鉴定
4. MRM QUANTIFICATION OF HOST CELL PROTEINS IN PROTEIN BIOPHARMACEUTICALS [C] . Catalin E. Doneanu, Weibin Chen, Alex Xenopoulos, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：蛋白质生物制药中宿主细胞蛋白的MRM定量
5. Arenavirus-Host Interactions: Roles of Viral Glycoprotein and Nucleoprotein in Mediating Cell Entry and Host Immune Suppression [D] . Shao, Junjie. 2018

机译：arenavirus-host相互作用：病毒糖蛋白和核蛋白在介质细胞进入和宿主免疫抑制中的作用
6. An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry [O] . Catalin Doneanu, Jing Fang, Yun Alelyunas, 2018

机译：通过液相色谱离子淌度QTOF质谱定量蛋白质生物制药中宿主细胞蛋白质的HS-MRM分析
7. An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry [O] . Catalin Doneanu, Jing Fang, Yun Alelyunas, 2018

机译：通过液相色谱离子迁移率QTOF质谱法定量蛋白质生物制药中宿主细胞蛋白的HS-MRM测定

MRM QUANTIFICATION OF HOST CELL PROTEINS IN PROTEIN BIOPHARMACEUTICALS

摘要

著录项

相似文献

相关主题

期刊订阅