Application of Sheathless CE-ESI-MS for the Identification of Phosphorylation Sites of Caldesmon after Incubation with Myosin Light Chain Kinase

机译：用肌蛋白轻链激酶孵育后鞘蛋白酶磷酸化位点鉴定的鞘CE-ESI-MS

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1 Several phosphopeptides were identified with CE-ESI-MS and LC-ESI-MS. 2 Applying the same sample amount 5 different phospho-peptides were found by CE-ESI-MS, 2 different phospho-peptides were found by LC-ESI-MS. 3 One novel phosphorylation site was identified, namely Thr-626. 4 Analysis time using CE is four times faster (9 min) compared to LC (35 min). 5 CE shows high resolution and efficiency, but is very sensitive to the buffer concentration used for enzymatic digestion. 6 Sheathless CE-ESI-MS with porous tip is very sensitive, no sample dilution by make-up flow.

机译：用CE-ESI-MS和LC-ESI-MS鉴定出几种磷酸肽。 2施加相同的样品量，通过CE-ESI-MS发现5种不同的磷酸肽，通过LC-ESI-MS发现2种不同的磷酸肽。 3鉴定了一种新型磷酸化位点，即Thr-626。与LC（35分钟）相比，4使用CE的分析时间速度快（9分钟）。 5 Ce显示出高分辨率和效率，但对用于酶消化的缓冲浓度非常敏感。 6具有多孔尖端的护套CE-ESI-MS非常敏感，通过化妆流动无样品稀释。

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《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2011年||共1页
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Bettina Sarg; Leopold Kremser; Apolinary Sobieszek; Bernhard Halfinger; Herbert Lindner;
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1. A subset of protein kinase C phosphorylation sites on the myosin II regulatory light chain inhibits phosphorylation by myosin light chain kinase. [J] . Turbedsky K, Pollard TD, Bresnick AR Biochemistry . 1997,第8期

机译：肌球蛋白II调节轻链上的蛋白激酶C磷酸化位点的子集抑制肌球蛋白轻链激酶的磷酸化。
2. Phosphorylation of caldesmon by myosin light chain kinase increases its binding affinity for phosphorylated myosin filaments. [J] . Sobieszek A, Sarg B, Lindner H, Biological chemistry . 2010,第9期

机译：肌球蛋白轻链激酶使卡尔德斯蒙磷酸化，增加了其对磷酸化肌球蛋白丝的结合亲和力。
3. Identification of novel in vitro protein kinase A phosphorylation sites on recombinant non-muscle myosin light chain kinase: nano-liquidchromatography tandem mass spectrometry methodology [J] . JING ZHAO, SARA M. CAMP, EDDIE T. CHIANG, Journal of organ dysfunction . 2009,第4期

机译：重组非肌肉肌球蛋白轻链激酶上的新型体外蛋白激酶A磷酸化位点的鉴定：纳米液相色谱串联质谱法
4. Application of Sheathless CE-ESI-MS for the Identification of Phosphorylation Sites of Caldesmon after Incubation with Myosin Light Chain Kinase [C] . Bettina Sarg, Leopold Kremser, Apolinary Sobieszek, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：用肌蛋白轻链激酶孵育后鞘蛋白酶磷酸化位点鉴定的鞘CE-ESI-MS
5. Mitogen-Activated Protein Kinase and Protein Kinase C-catalyzed caldesmon phosphorylation in vascular smooth muscle: Is it more important in the regulation of resting tone or stimulation-induced contraction? [D] . Trappanese, Danielle M. 2012

机译：丝裂素激活的蛋白激酶和蛋白激酶C催化的血管平滑肌Caldesmon磷酸化：在调节静息音或刺激引起的收缩中更重要吗？
6. Differential signalling by muscarinic receptors in smooth muscle: m2-mediated inactivation of myosin light chain kinase via Gi3 Cdc42/Rac1 and p21-activated kinase 1 pathway and m3-mediated MLC20 (20 kDa regulatory light chain of myosin II) phosphorylation via Rho-associated kinase/myosin phosphatase targeting subunit 1 and protein kinase C/CPI-17 pathway. [O] . Karnam S Murthy, Huiping Zhou, John R Grider, 2003

机译：平滑肌中毒蕈碱受体的差异信号：通过Gi3Cdc42 / Rac1和p21激活的激酶1途径m2介导的肌球蛋白轻链激酶的失活以及通过Rho的m3介导的MLC20（肌球蛋白II的20 kDa调节轻链）的磷酸化-相关激酶/肌球蛋白磷酸酶靶向亚基1和蛋白激酶C / CPI-17途径。
7. Differential signalling by muscarinic receptors in smooth muscle: m2-mediated inactivation of myosin light chain kinase via Gi3, Cdc42/Rac1 and p21-activated kinase 1 pathway, and m3-mediated MLC20 (20 kDa regulatory light chain of myosin II) phosphorylation via Rho-associated kinase/myosin phosphatase targeting subunit 1 and protein kinase C/CPI-17 pathway. [O] . Murthy, Karnam S, Zhou, Huiping, Grider, John R, 2003

机译：平滑肌中毒蕈碱受体的差异信号：通过Gi3，Cdc42 / Rac1和p21激活的激酶1途径，m2介导的肌球蛋白轻链激酶的失活，以及通过Rho的m3介导的MLC20（肌球蛋白II的20 kDa调节轻链）的磷酸化-相关激酶/肌球蛋白磷酸酶靶向亚基1和蛋白激酶C / CPI-17途径。
8. Myosin Light Chain Kinase Inhibitors and Methods of Use. [R] . Turner, J. R., Mrsny, R. J., McKay, D. 2005

机译：肌球蛋白轻链激酶抑制剂和使用方法。

Application of Sheathless CE-ESI-MS for the Identification of Phosphorylation Sites of Caldesmon after Incubation with Myosin Light Chain Kinase

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