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首页> 外文会议>American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics >Needle in the Haystack - Shotgun analysis of highly enriched phosphoproteins from Arabidopsis
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Needle in the Haystack - Shotgun analysis of highly enriched phosphoproteins from Arabidopsis

机译：Haystack中的针 - 来自拟南芥高度富集的磷蛋白的霰弹枪分析

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The poor accessibility to phosphoproteins notably low abundant ones gets researchers into trouble when looking for signalling components. With the establishment of this two step procedure (PAPE) we were able to highly enrich phosphor-proteins from complex leaf sample of Arabidopsis thaliana. The prefractionation step depleted almost all of the RuBisCO content and served as a first enrichment. Subsequent MOAC further increased the phosphoprotein content. Reproducibility could be visibly verified with a phosphospecific dye and more importantly via mass spectrometry. The detection of low abundant phosphoproteins, namely MPK6 and MPK4, could be achieved. The implementation of this method into our workflow of the artificial response system recently started and shows promising results. Future studies will focus on seeking for new MAPK3/6 targets with the help of the above system and the target confirmation.
机译：在寻找信令组件时，磷蛋白的可差的可达性尤其是低丰富的人在研究时将研究人员陷入困境。通过建立这两步程序（PAPE），我们能够从拟南芥的复杂叶样样品中获得高度丰富的磷光体蛋白。预提词步骤几乎耗尽了几乎所有的Rubisco含量，并作为第一个富集。随后的MOAC进一步增加了磷蛋白含量。可以用磷酸化染料明显地验证再现性，并且通过质谱法更重要。可以实现检测低丰富的磷蛋白，即MPK6和MPK4。该方法的实施成为我们最近开始的人工响应系统的工作流程，并显示了有希望的结果。未来的研究将借助上述系统和目标确认，专注于寻求新的MAPK3 / 6目标。

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