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首页> 外文会议>American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics >Peptidomic Survey of Secreted Peptide Hormones from Enteroendocrine Cells by Differential Mass Spectrometry
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Peptidomic Survey of Secreted Peptide Hormones from Enteroendocrine Cells by Differential Mass Spectrometry

机译：通过差分质谱法对肠内分泌细胞分泌肽激素的肽调查

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摘要

To investigate the mechanism underlying processing and release of incretins from enteroendocrine (EE) cells in the intestine, we performed a differential mass spectrometry (dMS) study to profile secreted peptide hormones from murine EE cell lines, GLUTag and STC-1, under basal and stimulated conditions. Secreted peptidomes in the cell media under the two conditions were analyzed on a nanoflow LC-FTMS platform; only peptides with elevated levels under stimulated condition were subjected to sequencing. Two important features of this approach, a system free of intracellular protein background and selection of precursor ions of secreted peptides, facilitated an efficient method for cataloging bioactive peptides. Consequently, high sequence coverage of several key prohormone sequences was achieved, providing insights into regulated hormone secretion from EE cells.
机译：为了探讨肠中肠内内分泌（EE）细胞的潜在处理和释放Incetetins的机制，我们进行了差分质谱（DMS）研究，以在基础和基础上的鼠EE细胞系，谷氨酸和STC-1分泌分泌的肽激素。刺激的条件。在纳米洛LC-FTMS平台上分析了在两个条件下细胞培养基中的分泌肽物;对刺激条件下具有升高水平的肽进行测序。这种方法的两个重要特征，一种没有细胞内蛋白质背景的系统以及分泌肽的前体离子的选择，促进了用于目录生物活性肽的有效方法。因此，实现了几个关键前谐波序列的高序列覆盖，从EE细胞中提供了对调节激素分泌的见解。

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