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首页> 外文会议>International Conference on Environmental Science and Technology >ANIMAL FEED PRODUCTION BY SOLID STATE FERMENTATION OF BREWER'S SPENT GRAINS AND MALT SPENT ROOTLETS WITH ASPERGILLUS AWAMORI, A. ORYZAE AND PHANEROCHAETE CHRYSOSPORIUM
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ANIMAL FEED PRODUCTION BY SOLID STATE FERMENTATION OF BREWER'S SPENT GRAINS AND MALT SPENT ROOTLETS WITH ASPERGILLUS AWAMORI, A. ORYZAE AND PHANEROCHAETE CHRYSOSPORIUM

机译：通过固态发酵的动物饲料生产Brewer的谷物和麦芽植物的玉米氏菌，A. Oryzae和Phanerochaete Chrysosporium

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Brewer's spent grains (BSG), malt spent rootlets (MSR) and their mixtures (BSG-MSR), with/without addition of molasses, orange and potato pulp, as additional carbon sources, were treated with the filamentous fungi A. awamori, A. oryzae, and the ligninolytic white-rot fungus Phanerochaete chrysosporium, for the production of protein enriched animal feeds. Various types of solid state fermentation (SSF) processes were carried out at 30°C. The fermented substrates had different initial moisture contents (60-80%) and different initial sugar concentrations. SSFs were carried out under aseptic conditions, in 3 types of bioreactors. All experiments were carried out without addition of extra nutrients. In the first case, SSFs of BSG, MSR and BSG-MSR, with A. awamori or A. oryzae were carried out with & without addition of a 10°Be solution of molasses (initial sugar concentration ~0.3g per 20g of substrate). SSFs were carried out in conical flasks (a) and in petri dishes (b). Both fungi performed well in all the tested systems, with high biomass yields within 3-4 days. SSFs were also carried out at larger scale, in glass-tower reactors (c). Each fermentation system contained 100g BSG, suitable amount of Aspergillus spore suspension and various amounts of molasses (10°Be), orange pulp (undiluted), potato pulp (1:1 with water) and finally mixtures of molasses, orange and potato pulps. Experiments were also carried out without addition of the above carbohydrate materials. The average real protein increase obtained in the above SSF systems after treatment with Aspergillus spores was about 35%. Fermentation times were lower in the case of bioreactor types (a) and (b), although the (c) type systems may allow larger scale applications, better moisture control and homogenous fungal growth within the fermented material bulk. In the second case, SSFs of BSG, MSR and BSG-MSR, with the white-rot, fungus P. chrysosporium, were carried out in petri dishes (b) with and without the addition of various amounts of molasses and orange pulp. Various initial moisture contents (40-80%) and initial sugar concentrations were evaluated. Fungal growth was also fast in all cases, with good protein increase levels as in the previous cases. Nevertheless, in all the studied systems, the fungi were unable to grow efficiently in the absence of extra fermentable sugars, as excepted. Fungal growth was higher in BSG compared to MSR, since MSR contain lower amounts of fermentable sugars or starch. All samples were readily accepted by chicken.
机译：用丝状真菌A. awamori治疗酿酒厂的谷物（BSG），麦芽废根（MSR）及其混合物（BSG-MSR），与/不添加糖蜜，橙色和马铃薯浆，作为额外的碳源。 。oryzae，和木质素溶解的白腐真菌植物，用于生产蛋白质富集的动物饲料。在30℃下进行各种类型的固态发酵（SSF）方法。发酵的底物具有不同的初始水分含量（60-80％）和不同的初始糖浓度。 SSFS在无菌条件下进行3种生物反应器进行。所有实验均进行所有实验，而无需添加额外的营养素。在第一种情况下，使用A. awamori或A. oryzae的BSG，MSR和BSG-MSR的SSFS与糖蜜溶液（每20g底物〜0.3g的初始糖浓度〜0.3g）进行。 。 SSFS在锥形烧瓶（A）和培养皿中进行（B）。在所有测试的系统中，均在所有测试系统中表现良好，在3-4天内具有高的生物量产量。 SSFS也以较大的尺寸进行，玻璃塔反应器（C）进行。每种发酵系统含有100g BSG，合适量的曲霉菌孢子悬浮液和各种量的糖蜜（10°是），橙色纸浆（未稀释），马铃薯纸浆（1：1用水），最后混合物的糖蜜，橙色和马铃薯浆混合物。还进行了实验而不加入上述碳水化合物材料。在用曲霉菌孢子处理后，在上述SSF系统中获得的平均实际蛋白质增加约为35％。在生物反应器类型（a）和（b）的情况下，发酵时间较低，尽管（c）型系统可以允许更大的规模应用，更好的水分控制和发酵材料体内的均匀真菌生长。在第二种情况下，BSG，MSR和BSG-MSR的SSF，用白色腐肉，真菌P.Chrysosporium，在培养皿（B）中进行，并且不添加各种量的糖蜜和橙色纸浆。评估各种初始含水量（40-80％）和初始糖浓度。在所有情况下，真菌生长也很快，蛋白质良好的蛋白质增加，如先前的病例。然而，在所有研究的系统中，真菌在没有额外的可发酵糖的情况下，真菌无法有效地生长。与MSR相比，BSG的真菌生长较高，因为MSR含有较少量的可发酵的糖或淀粉。鸡肉易于接受所有样品。

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