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首页> 外文会议>International Horticulture Congress on Science and Horticulture for People >Expression Profiles of Defense-Related Genes in Response to Aphid Inoculation in Different Chrysanthemum Cultivars
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Expression Profiles of Defense-Related Genes in Response to Aphid Inoculation in Different Chrysanthemum Cultivars

机译：国防相关基因的表达谱响应不同菊花品种蚜虫接种

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摘要

The expression profiles of genes involved in the defense enzyme system, lignin biosynthesis and flavonoids pathway encoding peroxidase (POD), polyphenoloxidase (PPO), phenylalanine ammonia lyase (PAL), catechol-o-methyltransferase (COMT), ferulate 5-hydroxylase (F5H), chalcone synthase (CHS) and dihydroflavonol reductase (DFR) were determined using real-time quantitative PCR in chrysanthemum cultivars differing in aphid resistance. The results indicated that POD, PPO, PAL, COMT and F5H were induced in highly aphid resistant 'Keiun', moderately resistant 'Han6' and susceptible 'Jinba', whereas CHS and DFR were induced only in two aphid resistant cultivars, but not in susceptible 'Jinba'. The transcripts of POD, PPO, PAL, COMT and F5H genes increased more rapidly and peaked earlier in the resistant 'Keiun' and 'Han6' than in the susceptible 'Jinba'. The different expression profiles of these genes in response to aphid inoculation between resistant and susceptible cultivars suggested that these genes probably play important roles in aphid resistance of chrysanthemum.
机译：涉及的基因的表达谱涉及防御酶系统，木质素生物合成和黄酮类化合物途径编码过氧化物酶（POD），多酚氧化酶（PPO），苯丙氨酸氨裂解酶（PAL），儿茶酚-O-甲基转移酶（COMT），发射5-羟基（F5H） ），使用在蚜虫抗性的菊花品种中的实时定量PCR测定Chalcode合酶（CHS）和二氢酰酰酚醇还原酶（DFR）。结果表明，在高度蚜虫的“Keiun”，中度抗性的'HAN6'和易感'Jinba'中诱导了POD，PPO，PAL，COMT和F5H，而CHS和DFR仅在两个蚜虫抗性品种中诱导，但不诱导易感'jinba'。 POD，PPO，PAL，COMT和F5H基因的成绩单更快地增加，在抗性'Keiun'和“Han6”中比易感'Jinba'更快地增加。这些基因的不同表达谱响应抗蚜虫和易感品种之间的蚜虫接种，表明这些基因可能在菊花的蚜虫抗蚜虫中起重要作用。

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