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Sensitive detection of interferon-gamma with engineered proteins and surface plasmon resonance biosensor

机译：具有工程蛋白质和表面等离子体共振生物传感器的干扰素-γ的敏感性检测

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We present a novel surface plasmon resonance (SPR) biosensor for detection of human interferon gamma (hIFNγ), an important diagnostic marker for several infectious diseases. The biosensor employs engineered proteins derived from albumin binding domain (ABD) and selected with ribosome display to provide high affinity towards hIFNγ. We demonstrate that the biosensor provides rapid, sensitive, and cost-effective platform for the detection of hIFNγ and allows its detection at nanomolar levels both in buffer and diluted human plasma.

机译：我们提出了一种新的表面等离子体共振（SPR）生物传感器，用于检测人干扰素γ（HIFNγ），这是几种传染病的重要诊断标志物。生物传感器采用衍生自白蛋白结合结构域（ABD）的工程化蛋白，并用核糖体显示器选择，以向HIFNγ提供高亲和力。我们证明，生物传感器为检测HIFNγ提供快速，敏感和经济效益的平台，并允许在缓冲液和稀释的人血浆中检测纳摩尔水平。

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《Anniversary Eurosensors Conference》|2013年||共4页
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作者
H. Sipova; V. Sevcu; M. Kuchar;
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中图分类 TP212.2;
关键词
Surface plasmon resonance; Biosensor; Scaffold; Engineered proteins; Interferon gamma;

机译：表面等离子体共振;生物传感器;脚手架;工程蛋白;干扰素伽玛;

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专利

1. Surface plasmon resonance biosensor based on engineered proteins for direct detection of interferon-gamma in diluted blood plasma [J] . H. Sipova, V. Sevcu, M. Kuchar, Sensors and Actuators . 2012,第期

机译：基于工程蛋白的表面等离子体共振生物传感器，可直接检测稀释血浆中的γ-干扰素
2. Au-Ag-Au double shell nanoparticles-based localized surface plasmon resonance and surface-enhanced Raman scattering biosensor for sensitive detection of 2-mercapto-1-methylimidazole [J] . Liao X., Qin M., Chen Y., Talanta: The International Journal of Pure and Applied Analytical Chemistry . 2013,第Null期

机译：基于Au-Ag-Au双壳纳米粒子的局域表面等离子体共振和表面增强拉曼散射生物传感器，用于2-巯基-1-甲基咪唑的灵敏检测
3. Chemisorptions of bacterial receptors for hydrophobic amino acids and sugars on gold for biosensor applications: a surface plasmon resonance study of genetically engineered proteins [J] . Luck LA., Moravan MJ., Garland JE., Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2003,第3期

机译：用于生物传感器的金上疏水氨基酸和糖的细菌受体的化学吸附：基因工程蛋白的表面等离子体共振研究
4. Sensitive detection of interferon-gamma with engineered proteins and surface plasmon resonance biosensor [C] . H. Sipova, V. Sevcu, M. Kuchar Anniversary Eurosensors Conference . 2013

机译：具有工程蛋白质和表面等离子体共振生物传感器的干扰素-γ的敏感性检测
5. Fabrication of a Surface Plasmon Resonance Platform for the Development of an Electrokintic Surface Plasmon Resonance (EK-SPR) Biosensor [D] . Sathoud, Ornella 2018

机译：表面等离子体共振平台的制造，用于开发电动表面等离子体共振（EK-SPR）生物传感器。
6. Development of a Molecular Imprinting-Based Surface Plasmon Resonance Biosensor for Rapid and Sensitive Detection of Staphylococcus aureus Alpha Hemolysin From Human Serum [O] . Tilde Andersson, Anna Bläckberg, Rolf Lood, 2020

机译：基于分子印迹的表面等离子体共振生物传感器的研制快速敏感检测人血清葡萄球菌α血糖素的快速和敏感性检测
7. Sensitive Detection of Interferon-Gamma with Engineered Proteins and Surface Plasmon Resonance Biosensor [O] . Šípová H., Ševců V., Kuchař M., 2011

机译：工程蛋白和表面等离振子共振生物传感器灵敏检测干扰素-γ

Sensitive detection of interferon-gamma with engineered proteins and surface plasmon resonance biosensor

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