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SNR characterization of Mesoscopic Fluorescence Molecular Tomography with EMCCD camera

机译:与EMCCD相机的脑镜荧光分子断层扫描的SNR表征

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Progress in bio-printing techniques has accentuated the need for non-invasive imaging modalities with high sensitivity, high resolution, and imaging depth of a few millimeters. Mesoscopic Fluorescence Molecular Tomography (MFMT) is a promising imaging modality for 3D localization and quantification of fluorescent labeled cells in thick scaffolds. Here we report on the characterization of our second generation MFMT system which uses an Electron-Multiplying charge coupled device (EMCCD). More precisely, we report on the effects of EM gain and deep cooling of the camera on signal-to-noise ratio (SNR) and hence the sensitivity. Experimental results showed that these parameters, when used in conjunction, increased SNR by at least 3 fold.
机译:生物印刷技术的进展已经强调了具有高灵敏度,高分辨率和成像深度的非侵入性成像模式的需求。介观荧光分子断层扫描(MFMT)是用于3D定位的有望的成像模态,并在厚支架中定量荧光标记的细胞。在这里,我们报告了我们的第二代MFMT系统的表征,该系统使用电子乘法电荷耦合器件(EMCCD)。更确切地说,我们报告了EM增益和深冷却相机对信噪比(SNR)的影响,因此灵敏度。实验结果表明,这些参数在结合使用时,增加了SNR至少3倍。

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