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Sterilization Method of Etlingera Elatior Explant on Tissue Culture

机译：Etlingera Elatior浅析组织培养的灭菌方法

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Etlingera elatior or in Sundanese known as honje is a type of traditional medicinal plant that is potential to be developed.Utilization of honje raw material as a medicinal plant is still harvested in nature without cultivation process.Traditionally honje is propagated through rhizomes with high infection rates because the rhizomes experience decay caused by bacteria of Phytium species.To overcome this problem,tissue culture as a conservation effort is needed to produce free pests and diseases seedlings.This study was aimed to find out explants that gave the best results on the sterilization of honje plants that would be used as planting material on cultivation with tissue culture.This research was conducted at Plant Tissue Culture Laboratory of Agrotechnology Dept.of Uin Sunan Gunung Djati Bandung.This study used two types of explants i.e.bud and bud base in the process of sterilization,each repeated 20 times.The results showed that the best explant on honje plant on sterilization was in the treatment of base of bud explant by showing the lowest contamination level with the number of live explant almost 88% and showed the beginning of shoot formation at age 3 DAI.
机译：茴香砂仁属丽格或巽称为honje是一种类型的传统药用植物即潜在是honje原料的developed.Utilization作为药用植物在自然界中仍然没有收获培养process.Traditionally honje通过与高感染率根茎传播因为由Phytium species.To的细菌根茎腐烂经验克服这个问题，组织文化作为保护工作的需要生产无病虫害seedlings.This研究旨在找出了对消毒的最好成绩植这将被用作种植材料栽培与组织culture.This研究honje植物在农业技术Dept.of UIN苏南火山Djati Bandung.This研究的植物组织培养实验室进行使用两种类型的外植体的过程中iebud和芽基地杀菌，每组重复20个。结果表明，在honje植物最好的植上了绝育手术在由显示用的外植活的数目几乎88％的最低污染水平并表现出枝条形成开始在3岁DAI治疗芽外植体的碱。

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