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首页> 外文会议>International Conference Interdisciplinarity in Engineering >Kinetics Properties of Marine Chitinase from Novel Red Sea Strain of Bacillus
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Kinetics Properties of Marine Chitinase from Novel Red Sea Strain of Bacillus

机译：芽孢杆菌新红海菌株海洋胰蛋白酶的动力学特性

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An aerobic Gram variable rod shaped polarly flagellated marine bacterium abbreviated R2 attracted our attention not by its hyper chitinolytic activity but also by its multiple enzymes production (protease, gelatinase, lipase, amylase, dextranase,alginase, arabinase, agarase, etc.). This bacterial isolate was selected and identified using conventional methods as well as 16S rRNA technique and submitted in the Gen Bank sequence database as Bacillus sp. R2 with a given accession number DQ 923161. Its purified chitinase showed a molecular weight of 41.68 KDa. Kinetics study revealed that the chitinase exhibited Km, Vmax and Kcat values of 6.971 mg/ml, 69.63 U/ml and 1.815 ncatal for colloidal chitin, 3.334 mg/ml, 83.32 U/ml and 2.172 ucatal for squid chitin respectively. Furthermore the enzyme catalytic efficiency (Kcat/Km) was estimated to be 0.260 and 0.651 μM P.min~(-1) mol~(-1)mg~(-1)S toward colloidal chitin and squid chitin, respectively. This is the first contribution about marine chitinase kinetics study from novel gram variable Bacillus isolated from the Red sea.
机译：一种有氧革克变形杆形绰号的船只缩写R2吸引了我们的注意力而不是通过其多胰蛋白酶产生（蛋白酶，明胶酶，脂肪酶，淀粉酶，葡聚糖酶，Alginase，阿拉伯酶，琼脂酶等）引起我们的注意。选择该细菌分离物并使用常规方法以及16S rRNA技术鉴定，并在Gen Bank序列数据库中提交为Bacillus SP。 R2具有给定的附加号DQ 923161.其纯化的逐蛋白酶显示出41.68kDa的分子量。动力学研究表明，胰蛋白酶分别表现出6.971mg / ml，69.63毫/ mL，69.63u / ml和1.815ncatal的KIM，VMAX和KCAT值，分别为鱿鱼壳蛋白的3.334mg / ml，83.32u / ml和2.172 ucatal。此外，鉴定酶催化效率（KCAT / Km）分别估计为0.260和0.651μmP.MIN〜（-1）mol〜（-1）mg〜（-1）mg〜（-1）s，朝向胶体丁蛋白和鱿鱼甲酸酯。这是从红海中分离的新型克变性芽孢杆菌的海洋胰蛋白酶动力学研究的第一种贡献。

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